Suppressors of SNF2 mutations restore invertase derepression and cause temperature-sensitive lethality in yeast

Mutations in the SNF2 gene of Saccharomyces cerevisiae prevent derepression of the SUC2 (invertase) gene, and other glucose-repressible genes, in response to glucose deprivation. We have isolated 25 partial phenotypic revertants of a snf2 mutant that are able to derepress secreted invertase. These r...

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Bibliographic Details
Published in:Genetics (Austin) 1986-04, Vol.112 (4), p.741-753
Main Authors: Neigeborn, L, Rubin, K, Carlson, M
Format: Article
Language:English
Subjects:
ACTIVIDAD ENZIMATICA
ACTIVITE ENZYMATIQUE
beta-Fructofuranosidase
Biological and medical sciences
CELLS
CELLULE
CELULAS
Classical genetics, quantitative genetics, hybrids
Enzyme Repression
ENZYMIC ACTIVITY
FRUCTOFURANOSIDASA
FRUCTOFURANOSIDASE
Fundamental and applied biological sciences. Psychology
GENE
GENES
Genes, Dominant
Genes, Lethal
Genes, Recessive
Genetic Complementation Test
Genetics of eukaryotes. Biological and molecular evolution
Genotype
Glycoside Hydrolases - biosynthesis
Glycoside Hydrolases - genetics
Investigations
MUTACION
MUTATION
Phenotype
SACCHAROMYCES CEREVISIAE
Saccharomyces cerevisiae - enzymology
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - radiation effects
Suppression, Genetic
TEMPERATURA
TEMPERATURE
Thallophyta, bryophyta
Ultraviolet Rays
Vegetals
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Summary:Mutations in the SNF2 gene of Saccharomyces cerevisiae prevent derepression of the SUC2 (invertase) gene, and other glucose-repressible genes, in response to glucose deprivation. We have isolated 25 partial phenotypic revertants of a snf2 mutant that are able to derepress secreted invertase. These revertants all carried suppressor mutations at a single locus, designated SSN20 (suppressor of snf2). Alleles with dominant, partially dominant and recessive suppressor phenotypes were recovered, but all were only partial suppressors of snf2, reversing the defect in invertase synthesis but not other defects. All alleles also caused recessive, temperature-sensitive lethality and a recessive defect in galactose utilization, regardless of the SNF2 genotype. No significant effect on SUC2 expression was detected in a wild-type (SNF2) genetic background. The ssn20 mutations also suppressed the defects in invertase derepression caused by snf5 and snf6 mutations, and selection for invertase-producing revertants of snf5 mutants yielded only additional ssn20 alleles. These findings suggest that the roles of the SNF2, SNF5 and SNF6 genes in regulation of SUC2 are functionally related and that SSN20 plays a role in expression of a variety of yeast genes.
ISSN:0016-6731
1943-2631