The naturally occurring alleles of MAL1 in Saccharomyces species evolved by various mutagenic processes including chromosomal rearrangement

In order for a yeast strain to ferment maltose it must contain any one of the five dominant MAL loci. Each dominant MAL locus thus far analyzed contains three genes: GENE 1, encoding maltose permease, GENE 2 encoding maltase and GENE 3 encoding a positive trans-acting regulatory protein. In addition...

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Bibliographic Details
Published in:Genetics (Austin) 1988-09, Vol.120 (1), p.83-93
Main Authors: Charron, M.J, Michels, C.A
Format: Article
Language:English
Subjects:
Alleles
Blotting, Southern
CHROMOSOME
Cloning, Molecular
CROMOSOMAS
DNA Restriction Enzymes
GENE
GENES
Genes, Fungal
Genes, Regulator
GENETICA
GENETIQUE
Investigations
Maltose - genetics
Membrane Transport Proteins - genetics
Monosaccharide Transport Proteins
MUTANT
MUTANTES
Mutation
Nucleotide Mapping
SACCHAROMYCES
Saccharomyces cerevisiae
Saccharomyces cerevisiae - genetics
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Summary:In order for a yeast strain to ferment maltose it must contain any one of the five dominant MAL loci. Each dominant MAL locus thus far analyzed contains three genes: GENE 1, encoding maltose permease, GENE 2 encoding maltase and GENE 3 encoding a positive trans-acting regulatory protein. In addition to these dominant MAL loci, several naturally occurring, partially functional alleles of MAL1 and MAL3 have been identified. Here, we present genetic and molecular analysis of the three partially functional alleles of MAL1: the MAL1p allele which can express only the MAL activator; the MAL1g allele which can express both a maltose permease and maltase; and the mal1(0) allele which can express only maltase. Based on our results, we propose that the MAL1p, MAL1g and mal1(0) alleles evolved from the dominant MAL1 locus by a series of rearrangements and/or deletions of this yeast telomere-associated locus as well as by other mutagenic processes of gene inactivation. One surprising finding is that the MAL1g-encoded maltose permease exhibits little sequence homology to the MAL1-encoded maltose permease though they appear to be functionally homologous
ISSN:0016-6731
1943-2631
1943-2631
DOI:10.1093/genetics/120.1.83