Suppressors of yeast actin mutations

Suppressors of a temperature-sensitive mutation (act1-1) in the single actin gene of Saccharomyces cerevisiae were selected that had simultaneously acquired a cold-sensitive growth phenotype. Five genes, called SAC (suppressor of actin) were defined by complementation tests; both suppression and col...

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Bibliographic Details
Published in:Genetics (Austin) 1989-04, Vol.121 (4), p.659-674
Main Authors: Novick, P, Osmond, B.C, Botstein, D
Format: Article
Language:English
Subjects:
Actins - genetics
beta-Fructofuranosidase
Biological and medical sciences
BIOSINTESIS
BIOSYNTHESE
Chitin - analysis
Chromosome Mapping
Classical genetics, quantitative genetics, hybrids
Cloning, Molecular
Cold Temperature
CONTROL GENETICO
Culture Media
Cytoskeleton
ESTRUCTURA CELULAR
Fluorescent Antibody Technique
Fundamental and applied biological sciences. Psychology
GENE
GENES
Genes, Dominant
Genes, Fungal
Genetic Complementation Test
Genetics of eukaryotes. Biological and molecular evolution
Glycoside Hydrolases - secretion
Investigations
LUTTE GENETIQUE
Microscopy, Electron
MUTANT
MUTANTES
Mutation
Phenotype
Plasmids
PROTEINAS
PROTEINE
SACCHAROMYCES CEREVISIAE
Saccharomyces cerevisiae - genetics
STRUCTURE CELLULAIRE
Suppression, Genetic
Thallophyta, bryophyta
ULTRAESTRUCTURA
ULTRASTRUCTURE
Vegetals
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Description
Summary:Suppressors of a temperature-sensitive mutation (act1-1) in the single actin gene of Saccharomyces cerevisiae were selected that had simultaneously acquired a cold-sensitive growth phenotype. Five genes, called SAC (suppressor of actin) were defined by complementation tests; both suppression and cold-sensitive phenotypes were recessive. Three of the genes (Sac1, SAC2 and SAC3) were subjected to extensive genetic and phenotypic analysis, including molecular cloning. Suppression was found to be allele-specific with respect to actin alleles. The sac mutants, even in ACT1+ genetic backgrounds, displayed phenotypes similar to those of actin mutants, notably aberrant organization of intracellular actin and deposition of chitin at the cell surface. These results are interpreted as being consistent with the idea that the SAC genes encode proteins that interact with actin, presumably as components or controllers of the assembly or stability of the yeast actin cytoskeleton. Two unexpected properties of the SAC1 gene were noted. Disruptions of the gene indicated that its function is essential only at temperatures below about 17 degrees and all sac1 alleles are inviable when combined with act1-2. These properties are interpreted in the context of the evolution of the actin cytoskeleton of yeast
ISSN:0016-6731
1943-2631
1943-2631
DOI:10.1093/genetics/121.4.659