Meiotic recombination-deficient mutants of Schizosaccharomyces pombe

A mutant screen employing the ade6-M26 recombination hotspot was developed and used to isolate Schizosaccharomyces pombe mutants deficient in meiotic recombination. Nine rec mutations were recessive, defining six complementation groups, and reduced ade6 meiotic recombination 3-fold to greater than o...

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Bibliographic Details
Published in:Genetics (Austin) 1989-09, Vol.123 (1), p.45-54
Main Authors: Ponticelli, A.S. (Fred Hutchinson Cancer Research Center, Seattle, WA), Smith, G.R
Format: Article
Language:English
Subjects:
Biological and medical sciences
Classical genetics, quantitative genetics, hybrids
Crosses, Genetic
Fundamental and applied biological sciences. Psychology
GENETICA
Genetics of eukaryotes. Biological and molecular evolution
GENETIQUE
Investigations
MEIOSE
MEIOSIS
MUTANT
MUTANTES
Mutation
Nitrosoguanidines - pharmacology
Plasmids
RECOMBINACION
RECOMBINAISON
Recombination, Genetic
SACCHAROMYCES
Saccharomycetales - genetics
Schizosaccharomyces - genetics
Spores - physiology
Thallophyta, bryophyta
Transformation, Genetic
Vegetals
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Summary:A mutant screen employing the ade6-M26 recombination hotspot was developed and used to isolate Schizosaccharomyces pombe mutants deficient in meiotic recombination. Nine rec mutations were recessive, defining six complementation groups, and reduced ade6 meiotic recombination 3-fold to greater than or equal to 300-fold when homozygous. Three recessive rec mutations analyzed further also reduced meiotic intragenic recombination at ura4 on chromosome III and intergenic recombination between pro2 and arg3 on chromosome I. The observed non-co-ordinate reductions of the recombinant frequencies in the three test intervals suggest a degree of locus (or intragenic vs. intergenic) specificity of the corresponding rec+ gene products. None of the mutations specifically inactivated the ade6-M26 hotspot. Additional rec genes may be identified with these methods
ISSN:0016-6731
1943-2631
1943-2631
DOI:10.1093/genetics/123.1.45