Analysis of URS(G)-Mediated Glucose Repression of the GAL1 Promoter of Saccharomyces cerevisiae

Repression of GAL1 expression during growth on glucose is mediated in part by cis-acting promoter elements designated URSG. We show that oligonucleotides containing sequences from two regions of URSG confer glucose repression upon a heterologous promoter. Repression caused by URSG is dependent on tr...

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Bibliographic Details
Published in:Genetics (Austin) 1992-02, Vol.130 (2), p.295-304
Main Authors: Flick, J. S, Johnston, M
Format: Article
Language:English
Subjects:
Genetics
Investigations
Sugar
Yeast
Online Access:Get full text
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Summary:Repression of GAL1 expression during growth on glucose is mediated in part by cis-acting promoter elements designated URSG. We show that oligonucleotides containing sequences from two regions of URSG confer glucose repression upon a heterologous promoter. Repression caused by URSG is dependent on trans-acting factors of the glucose repression pathway and is independent of orientation or location within a promoter, suggesting that URSG contains binding sites for a glucose-activated repressor protein(s). Genetic analysis identified three apparently novel genes (URR1, URR3 and URR4) that are specifically required for URSG-mediated repression and may encode such repressor proteins. Mutations in the URR genes suppress the defect in URSG derepression caused by a snf1 mutation.
ISSN:0016-6731
1943-2631