Insertional mutagenesis of the maize P gene by intragenic transposition of Ac

The P-rr allele of the maize P gene regulates the synthesis of pigments derived from flavan-4-ol in the pericarp, cob glumes and other floral organs. We characterized 21 P alleles derived by intragenic transposition of Ac from three known positions. Ac transpositions can occur in either direction in...

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Bibliographic Details
Published in:Genetics (Austin) 1992-05, Vol.131 (1), p.199-209
Main Authors: Athma, P. (Cold Spring Harbor Laboratory, Cold Spring Harbor, NY), Grotewold, E, Peterson, T
Format: Article
Language:English
Subjects:
Base Sequence
Biological and medical sciences
Classical genetics, quantitative genetics, hybrids
Cloning, Molecular
DNA Transposable Elements
EXPRESION GENICA
EXPRESSION DES GENES
Flowers & plants
Fundamental and applied biological sciences. Psychology
GENE
Gene Expression
GENES
GENETICA
Genetics
Genetics of eukaryotes. Biological and molecular evolution
GENETIQUE
GLUMA
GLUME
Investigations
Molecular Sequence Data
MUTACION
Mutagenesis, Site-Directed
MUTATION
NUCLEOTIDE
NUCLEOTIDOS
PERICARPE
PERICARPIO
PIGMENTACION
PIGMENTATION
Restriction Mapping
Substrate Specificity
Thallophyta, bryophyta
Vegetals
ZEA MAYS
Zea mays - genetics
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Summary:The P-rr allele of the maize P gene regulates the synthesis of pigments derived from flavan-4-ol in the pericarp, cob glumes and other floral organs. We characterized 21 P alleles derived by intragenic transposition of Ac from three known positions. Ac transpositions can occur in either direction in the P gene, and with no apparent minimum distance: in one case Ac transposed just 6 bp from its original insertion site. However, the distribution of transposed Ac elements was markedly nonrandom: of 19 transposed Ac elements derived from a single Ac donor, 15 were inserted in a 1.1-kb region at the 5' end of P, while none had inserted in an adjacent 3.2-kb intronic region. All of the Ac insertions affect both pericarp and cob glume pigmentation, providing further evidence that the P-rr allele contains a single gene required for both pericarp and cob glume pigmentation. The distribution of the inserted Ac elements and the phenotype conditioned by each allele suggests a structure of P-rr which is similar to that previously determined molecularly. Possible explanations for the nonrandom distribution of transposed Ac elements are discussed
ISSN:0016-6731
1943-2631
1943-2631
DOI:10.1093/genetics/131.1.199