Deletion of a single-copy tRNA affects microtubule function in Saccharomyces cerevisiae

• Published in: Genetics (Austin) 1993-12, Vol.135 (4), p.955-962
• Main Authors: Reijo, R. A, Cho, D. S, Huffaker, T. C
• Format: Article
• Language: English
• Subjects: Alleles; arginina; arginine; arn de transferencia; arn de transfert; arn mensajero; arn messager; Base Sequence; Biological and medical sciences; Classical genetics, quantitative genetics, hybrids; Codon; DNA Primers; expresion genica; expression des genes; Fundamental and applied biological sciences. Psychology; gene; Gene Deletion; gene expression; gene interaction; genes; Genes, Fungal; genetica; genetics; Genetics of eukaryotes. Biological and molecular evolution; genetique; glicoproteinas; glycoproteine; glycoproteins; induced mutation; interaccion de genes; interaction genique; Investigations; loci; locus; messenger rna; Microtubules - physiology; Molecular Sequence Data; mutacion inducida; Mutation; mutation provoquee; Phenotype; Ribonucleic acid; RNA; RNA, Transfer - genetics; Saccharomyces cerevisiae; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - ultrastructure; Suppression, Genetic; temperatura; temperature; Thallophyta, bryophyta; transfer rna; Vegetals; Yeast
• ISSN: 0016-6731; 1943-2631; 1943-2631
• DOI: 10.1093/genetics/135.4.955

rts1-1 was identified as an extragenic suppressor of tub2-104, a cold-sensitive allele of the sole gene encoding beta-tubulin in the yeast, Saccharomyces cerevisiae. In addition, rts1-1 cells are heat sensitive and resistant to the microtubule-destabilizing drug, benomyl. The rts1-1 mutation is a deletion of approximately 5 kb of genomic DNA on chromosome X that includes one open reading frame and three tRNA genes. Dissection of this region shows that heat sensitivity is due to deletion of the open reading frame (HIT1). Suppression and benomyl resistance are caused by deletion of the gene encoding a tRNA(Arg/AGG) (HSX1). Northern analysis of rts1-1 cells indicates that HSX1 is the only gene encoding this tRNA. Deletion of HSX1 does not suppress the tub2-104 mutation by misreading at the AGG codons in TUB2. It also does not suppress by interfering with the protein arginylation that targets certain proteins for degradation. These results leave open the prospect that this tRNA(Arg/AGG) plays a novel role in the cell.