The Yak1 protein kinase of Saccharomyces cerevisiae moderates thermotolerance and inhibits growth by an Sch9 protein kinase-independent mechanism

The growth defect associated with the loss of yeast A kinase activity can be alleviated by the overexpression or deletion of two other kinases, Sch9 and Yak1, respectively. Using tests of epistasis, we have shown that Sch9 and Yak1 define separate signaling pathways and must, therefore, suppress the...

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Bibliographic Details
Published in:Genetics (Austin) 1994-02, Vol.136 (2), p.465-474
Main Authors: Hartley, A.D, Ward, M.P, Garrett, S
Format: Article
Language:English
Subjects:
Biological and medical sciences
CALOR
Cell Cycle
Cellular biology
CHALEUR
Classical genetics, quantitative genetics, hybrids
Cyclic AMP-Dependent Protein Kinases - genetics
Cyclic AMP-Dependent Protein Kinases - metabolism
Fundamental and applied biological sciences. Psychology
Fungal Proteins - metabolism
Galactose - pharmacology
GENE
Gene Expression Regulation, Fungal - drug effects
GENES
Genes, Fungal
Genetics
Genetics of eukaryotes. Biological and molecular evolution
Hot Temperature
Intracellular Signaling Peptides and Proteins
Investigations
LOCALISATION DE GENE
LOCALIZACION DE GENES
MUTACION INDUCIDA
Mutagenesis
MUTATION PROVOQUEE
Phenotype
Phosphorylation
Promoter Regions, Genetic
Protein Kinases - physiology
Protein-Serine-Threonine Kinases
PROTEINA QUINASA
PROTEINE KINASE
Recombinant Fusion Proteins - metabolism
RESISTANCE A LA TEMPERATURE
RESISTENCIA A LA TEMPERATURA
SACCHAROMYCES CEREVISIAE
Saccharomyces cerevisiae - enzymology
Saccharomyces cerevisiae - growth & development
Saccharomyces cerevisiae - physiology
Saccharomyces cerevisiae Proteins
Sequence Deletion
Thallophyta, bryophyta
Vegetals
Yeast
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Summary:The growth defect associated with the loss of yeast A kinase activity can be alleviated by the overexpression or deletion of two other kinases, Sch9 and Yak1, respectively. Using tests of epistasis, we have shown that Sch9 and Yak1 define separate signaling pathways and must, therefore, suppress the A kinase defect by different mechanisms. Nevertheless, the Yak1 kinase appears to regulate cellular processes that are under A kinase control. For example, acquisition of heat resistance is correlated with Yak1 kinase activity, such that Yak1-overexpressing cells are over 200-fold more resistant than isogenic yak1 strains. These results, for the first time, associate a phenotype, other than suppression of the A kinase growth defect, with the loss of Yak1 activity and argue a broader role for the Yak1 kinase in cell growth
ISSN:0016-6731
1943-2631
1943-2631
DOI:10.1093/genetics/136.2.465