Mutations in the Yeast PDR3, PDR4, PDR7 and PDR9 Pleiotropic (Multiple) Drug Resistance Loci Affect the Transcript Level of an ATP Binding Cassette Transporter Encoding Gene, PDR5

The yeast pleiotropic (multiple drug) resistance gene PDR5 encodes a product with homology to a large number of membrane transport proteins including the mammalian multiple drug resistance family. In this study, we identified four genes on chromosome II that affect the steady-state level of PDR5 tra...

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Bibliographic Details
Published in:Genetics (Austin) 1994-02, Vol.136 (2), p.505-515
Main Authors: Dexter, D, Moye-Rowley, W. S, Wu, A. L, Golin, J
Format: Article
Language:English
Subjects:
Adenosine Triphosphate - metabolism
Alleles
ATP-Binding Cassette Transporters
Biochemistry
Biological Transport, Active
Carrier Proteins - genetics
Carrier Proteins - metabolism
Chromosome Mapping
Chromosomes, Fungal
Cycloheximide - pharmacology
DNA-Binding Proteins - genetics
DNA-Binding Proteins - metabolism
Drug Resistance, Microbial - genetics
Fungal Proteins - genetics
Fungal Proteins - metabolism
Gene Expression Regulation, Fungal
Genes
Genes, Fungal
Genes, Regulator
Investigations
Leucine Zippers - genetics
Membrane Proteins - genetics
Membrane Proteins - metabolism
Mutation
Saccharomyces cerevisiae
Saccharomyces cerevisiae - drug effects
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - metabolism
Saccharomyces cerevisiae Proteins
Sequence Deletion
Trans-Activators
Transcription Factors - genetics
Transcription Factors - metabolism
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Description
Summary:The yeast pleiotropic (multiple drug) resistance gene PDR5 encodes a product with homology to a large number of membrane transport proteins including the mammalian multiple drug resistance family. In this study, we identified four genes on chromosome II that affect the steady-state level of PDR5 transcript in addition to a previously identified positive regulator, PDR1. The genes in question are PDR3, PDR4, PDR7 and PDR9. We also analyzed the interaction between PDR5 and YAP1. YAP1 encodes a positive regulator with a leucine zipper motif that causes pleiotropic drug resistance when overproduced. YAP1-mediated pleiotropic drug resistance is not dependent on the presence of PDR5 and must act through other genes.
ISSN:0016-6731
1943-2631
1943-2631
DOI:10.1093/genetics/136.2.505