Involvement of the host initiator function dnaA in the replication of coliphage lambda

• Published in: Genetics (Austin) 1995-04, Vol.139 (4), p.1469-1481
• Main Authors: Wegrzyn, G, Wegrzyn, A, Konieczny, I, Bielawski, K, Konopa, G, Obuchowski, M, Helinski, D R, Taylor, K
• Format: Article
• Language: English
• Subjects: Bacterial Proteins - physiology; Bacteriophage lambda - genetics; Deoxyribonucleic acid; DNA; DNA Replication - genetics; DNA, Viral - biosynthesis; DNA-Binding Proteins - physiology; Genetics; GTP-Binding Proteins - genetics; Investigations; rac GTP-Binding Proteins; Recombination, Genetic; Replication Origin
• ISSN: 0016-6731; 1943-2631; 1943-2631
• DOI: 10.1093/genetics/139.4.1469

We demonstrate that the initiation of coliphage lambda DNA replication is dependent on the host initiator function dnaA, provided that the lambdoid prophage Rac is absent. Presence of Rac compensated the absence of dnaA function, causing initiation of replication. In dnaAts rac+ cells at 43 degrees, most of parental phage DNA molecules, after one round of theta replication, switched to a replication with features of the sigma mode and produced progeny at high yield. Initiation of replication of the lambda Pts1 mutant at 43 degrees was blocked by dnaA function; however, under dnaA-rac+ conditions all parental phage DNA molecules, after one round of theta replication, switched to the sigma mode and produced progeny at high yield. Taking into account our recent finding that transcriptional activation of ori lambda seems to be dnaA-regulated (to be published elsewhere), we suggest that the DnaA-lambda Pts1 incompatibility occurs at the insertion of the ori lambda-bound lambda O-lambda P-DnaB preprimosome between the complementary lambda DNA strands. The role of Rac and the mechanism of the switch from theta to sigma mode of lambda phage DNA replication are discussed.