FRACTIONATION OF THYMIDINE PHOSPHOKINASE, THYMIDINE 5'-MONOPHOSPHATE PHOSPHOKINASE AND THYMIDINE 5'-DIPHOSPHATE PHOSPHOKINASE IN EXTRACTS OF LANDSCHUTZ ASCITES-TUMOUR CELLS

1. Extracts of Landschutz ascites-tumour cells have been fractionated by treatment with acid, alumina C(gamma) gel and Sephadex G-100 to yield purified preparations of thymidine phosphokinase, thymidine 5'-monophosphate phosphokinase and thymidine 5'-diphosphate phosphokinase. 2. These res...

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Bibliographic Details
Published in:Biochemical journal 1965-03, Vol.94 (3), p.518-524
Main Authors: GRAV, H J, SMELLIE, R M
Format: Article
Language:English
Subjects:
Ascites
Biochemical Phenomena
Biochemistry
Chemistry Techniques, Analytical
Chromatography
Chromatography, Gel
Dextrans
Neoplasms
Nucleoside-Phosphate Kinase
Nucleotides
Old Medline
Phosphotransferases
Spectrophotometry
Thymidine
Thymidine Kinase
Thymine Nucleotides
Tissue Culture Techniques
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Summary:1. Extracts of Landschutz ascites-tumour cells have been fractionated by treatment with acid, alumina C(gamma) gel and Sephadex G-100 to yield purified preparations of thymidine phosphokinase, thymidine 5'-monophosphate phosphokinase and thymidine 5'-diphosphate phosphokinase. 2. These results clearly demonstrate the existence in Landschutz ascites tumour of three phosphokinases each of which catalyses one step in the reaction sequence: thymidineright harpoon over left harpoonthymidine 5'-monophosphateright harpoon over left harpoonthymidine 5'-diphosphateright harpoon over left harpoonthymidine 5'-triphosphate. Though these results do not preclude the participation of other enzymes in the formation of thymidine 5'-triphosphate from thymidine by Landschutz ascites-tumour cells, they provide strong support for the view that thymidine 5'-diphosphate is an intermediate in the formation of thymidine 5'-triphosphate from thymidine 5'-monophosphate by this system.
ISSN:0264-6021
1470-8728
DOI:10.1042/bj0940518