Germline and Somatic Transformation of Mating Tetrahymena thermophila by Particle Bombardment

Mating Tetrahymena thermophila were bombarded with ribosomal DNA-coated particles at various times in development. Both macronuclear and micronuclear transformants were recovered. Optimal developmental stages for transformation occurred during meiosis for the micronucleus and during anlagen formatio...

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Bibliographic Details
Published in:Genetics (Austin) 1997-05, Vol.146 (1), p.135-147
Main Authors: Cassidy-Hanley, D, Bowen, J, Lee, J. H, Cole, E, VerPlank, L. A, Gaertig, J, Gorovsky, M. A, Bruns, P. J
Format: Article
Language:English
Subjects:
Animals
Deoxyribonucleic acid
Diploidy
DNA
DNA, Ribosomal
Genetic Vectors
Genetics
Germ-Line Mutation
Investigations
Microorganisms
Plasmids
Recombination, Genetic
Tetrahymena thermophila
Tetrahymena thermophila - genetics
Transformation, Genetic
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Summary:Mating Tetrahymena thermophila were bombarded with ribosomal DNA-coated particles at various times in development. Both macronuclear and micronuclear transformants were recovered. Optimal developmental stages for transformation occurred during meiosis for the micronucleus and during anlagen formation for the macronucleus. Evidence is given for transient retention of the introduced plasmid. Genetic and molecular tests confirmed that sexually heritable transformation was associated with integration at the homologous site in the recipient micronuclear chromosome.
ISSN:0016-6731
1943-2631
1943-2631
DOI:10.1093/genetics/146.1.135