Isolation and characterization of a temperature-sensitive circadian clock mutant of Neurospora crassa

A new circadian clock mutant has been isolated in Neurospora crassa. This new mutation, called period-6 (prd-6), has two features novel to known clock mutations. First, the mutation is temperature sensitive. At restrictive temperatures (above 21 degrees) the mutation shortens circadian period length...

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Bibliographic Details
Published in:Genetics (Austin) 1997-06, Vol.146 (2), p.525-530
Main Authors: Morgan, L.W, Feldman, J.F
Format: Article
Language:English
Subjects:
Biological Clocks - genetics
BIOLOGICAL RHYTHMS
CARTE GENETIQUE
Chromosome Mapping
CIRCADIAN RHYTHM
Circadian Rhythm - genetics
CONIDIATION
DOMINANCE
EPISTASIS
Epistasis, Genetic
ESPORULACION
FENOTIPOS
Fungi
GENE INTERACTION
Genes, Fungal
GENETIC MAPPING
GENETIC MAPS
Genetics
GROWTH RATE
INDICE DE CRECIMIENTO
INTERACCION DE GENES
INTERACTION GENIQUE
Investigations
MAPAS GENETICOS
MICELIO
MUTACION
MUTANT
MUTANTES
MUTANTS
MUTATION
MYCELIUM
NEUROSPORA CRASSA
Neurospora crassa - genetics
Neurospora crassa - growth & development
Neurospora crassa - physiology
PHENOTYPE
PHENOTYPES
RITMOS BIOLOGICOS
RYTHME BIOLOGIQUE
SPORULATION
TAUX DE CROISSANCE
TEMPERATURA
TEMPERATURE
TEMPS
TIEMPO
TIME
Time Factors
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Description
Summary:A new circadian clock mutant has been isolated in Neurospora crassa. This new mutation, called period-6 (prd-6), has two features novel to known clock mutations. First, the mutation is temperature sensitive. At restrictive temperatures (above 21 degrees) the mutation shortens circadian period length from a wild-type value of 21.5 hr to 18 hr. At permissive temperatures (below 21 degrees) the mutant has a 20.5-hr period length close to that of the wild-type strain. Second, the prd-6 mutation is epistatic to the previously isolated clock mutation period-2 (prd-2). This epistasis is unusual in that the prd-2 prd-6 double mutant strain has an 18-hr period length at both the restrictive and permissive temperatures. That is, the temperature-sensitive aspect of the phenotype of the prd-6 strain is lost in the prd-2 prd-6 double mutant strain. This suggests that the gene products of the prd-2 and prd-6 loci may interact physically and that the presence of a normal prd-2+ protein is required for low temperature to "rescue" the prd-6 mutant phenotype. These results, combined with our recent finding that prd-2 and some alleles of the frq gene show genetic synergy, suggest that it may be possible to establish a more comprehensive model of the Neurospora circadian clock.
ISSN:0016-6731
1943-2631
1943-2631
DOI:10.1093/genetics/146.2.525