Large scale identification of genes involved in cell surface biosynthesis and architecture in Saccharomyces cerevisiae

The sequenced yeast genome offers a unique resource for the analysis of eukaryotic cell function and enables genome-wide screens for genes involved in cellular processes. We have identified genes involved in cell surface assembly by screening transposon-mutagenized cells for altered sensitivity to c...

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Bibliographic Details
Published in:Genetics (Austin) 1997-10, Vol.147 (2), p.435-450
Main Authors: Lussier, M. (McGill University, Montreal, Quebec, Canada.), White, A.M, Sheraton, J, Di Paolo, T, Treadwell, J, Southard, S.B, Horenstein, C.I, Chen-Weiner, J, Ram, A.F.J, Kapteyn, J.C
Format: Article
Language:English
Subjects:
ANTIBIOTICOS
ANTIBIOTICS
ANTIBIOTIQUE
BETA GLUCANASA
BETA GLUCANASE
BIOSINTESIS
BIOSYNTHESE
BIOSYNTHESIS
CAFEINA
CAFEINE
CAFFEINE
CALCOFLUOR WHITE
Cell Membrane - metabolism
CELL MEMBRANES
CELL WALLS
Cellular biology
COMPOSE ORGANIQUE
COMPUESTOS ORGANICOS
DNA Transposable Elements
ENSAYO
FENOTIPOS
GENE
GENES
Genes, Fungal
Genetics
GLICOLIPIDOS
GLYCOLIPIDE
GLYCOLIPIDS
HIDROLASAS
HYDROLASE
HYDROLASES
HYGROMYCIN B
IDENTIFICACION
IDENTIFICATION
INDUCED MUTATION
Investigations
MEMBRANAS CELULARES
MEMBRANE CELLULAIRE
Microbial Sensitivity Tests
MUTACION
MUTACION INDUCIDA
MUTAGENESIS
Mutagenesis, Site-Directed
MUTANT
MUTANTES
MUTANTS
MUTATION
MUTATION PROVOQUEE
ORGANIC COMPOUNDS
PAPULACANDIN B
PARED CELULAR
PAROI CELLULAIRE
PHENOTYPE
PHENOTYPES
PLASMA MEMBRANES
PROTEINAS
PROTEINE
PROTEINS
SACCHAROMYCES CEREVISIAE
Saccharomyces cerevisiae - genetics
SCREENING
SUSCEPTIBILITY
TESTAGE
TESTING
TRANSPOSABLE ELEMENTS
Yeast
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Summary:The sequenced yeast genome offers a unique resource for the analysis of eukaryotic cell function and enables genome-wide screens for genes involved in cellular processes. We have identified genes involved in cell surface assembly by screening transposon-mutagenized cells for altered sensitivity to calcofluor white, followed by supplementary screens to further characterize mutant phenotypes. The mutated genes were directly retrieved from genomic DNA and then matched uniquely to a gene in the yeast genome database. Eighty-two genes with apparent perturbation of the cell surface were identified, with mutations in 65 of them displaying at least one further cell surface phenotype in addition to their modified sensitivity to calcofluor. Fifty of these genes were previously known, 17 encoded proteins whose function could be anticipated through sequence homology or previously recognized phenotypes and 15 genes had no previously known phenotype.
ISSN:0016-6731
1943-2631
1943-2631
DOI:10.1093/genetics/147.2.435