Loading…

The accumulation of P-element-induced recombinants in the germline of male Drosophila melanogaster

P-element-induced recombination in Drosophila melanogaster occurs premeiotically. Recombinants are therefore expected to accumulate in the stem cells of the germline of P-element-carrying males. We show that both the recombination frequency and the incidence of "clustering" increase with t...

Full description

Saved in:
Bibliographic Details
Published in:Genetics (Austin) 1997-12, Vol.147 (4), p.1769-1782
Main Authors: Tanaka, M.M. (Stanford University, CA.), Liang, X.M, Gray, Y.H.M, Sved, J.A
Format: Article
Language:English
Subjects:
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:P-element-induced recombination in Drosophila melanogaster occurs premeiotically. Recombinants are therefore expected to accumulate in the stem cells of the germline of P-element-carrying males. We show that both the recombination frequency and the incidence of "clustering" increase with the age of males carrying various P-element derivatives. The combination of end-deleted elements can lead to average recombination frequencies >50% with individual instances of 100% recombination. These elements also lowered the fertility of the carriers. We investigated these features by constructing an analytical and a computer simulation model of the course of events in the germline, incorporating the recently proposed hybrid element insertion (HEI) model of P-element activity. The model is able to predict extreme recombination levels, segregation ratio biases and lowered fertility through cell death in a single analysis.
ISSN:0016-6731
1943-2631
1943-2631
DOI:10.1093/genetics/147.4.1769