Calmodulin point mutations affect drosophila development and behavior

Calmodulin (CAM) is recognized as a major intermediary in intracellular calcium signaling, but as yet little is known of its role in developmental and behavioral processes. We have generated and studied mutations to the endogenous Cam gene of Drosophila melanogaster that change single amino acids wi...

Full description

Saved in:
Bibliographic Details
Published in:Genetics (Austin) 1997-12, Vol.147 (4), p.1783-1798
Main Authors: Nelson, H.B. (Lark Technologies, Inc., Houston, TX.), Heiman, R.G, Bolduc, C, Kovalick, G.E, Whitley, P, Stern, M, Beckingham, K
Format: Article
Language:English
Subjects:
ABNORMALITIES
AILE
ALAS
ALLELES
Amino Acid Sequence
ANIMAL CUTICLE
Animals
Behavior, Animal
BIOLOGICAL DEVELOPMENT
BLOOD VEINS
CALMODULIN
Calmodulin - chemistry
Calmodulin - genetics
CALMODULINA
CALMODULINE
CAM GENE
Chromosome Mapping
COMPLEMENTATION
CUTICULA ANIMAL
CUTICULE ANIMALE
DESARROLLO BIOLOGICO
DEVELOPPEMENT BIOLOGIQUE
DROSOPHILA MELANOGASTER
Drosophila melanogaster - genetics
Female
FENOTIPOS
FLIGHT
GENE
GENE LETAL
GENES
GENES LETALES
Genes, Lethal
GENETIC DISORDERS
GENETICA
GENETICS
GENETIQUE
INDUCED MUTATION
Insect Proteins - genetics
Insects
Investigations
LETHAL GENES
LOCOMOCION
LOCOMOTION
Male
MELANOSIS
MELANOTIC SCABS
Molecular Sequence Data
Molecular Structure
MUTACION INDUCIDA
MUTANT
MUTANTES
MUTANTS
Mutation
MUTATION PROVOQUEE
PHENOTYPE
PHENOTYPES
PIGMENTATION DISORDERS
Point Mutation
Space life sciences
STRUCTURAL GENES
TRASTORNOS DE LA PIGMENTACION
TRASTORNOS GENETICOS
TROUBLE DE LA PIGMENTATION
TROUBLE GENETIQUE
VEINE
VENAS
VIABILIDAD
VIABILITE
VIABILITY
VOL
VUELO
WINGS
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Calmodulin (CAM) is recognized as a major intermediary in intracellular calcium signaling, but as yet little is known of its role in developmental and behavioral processes. We have generated and studied mutations to the endogenous Cam gene of Drosophila melanogaster that change single amino acids within the protein coding region. One of these mutations produces a striking pupal lethal phenotype involving failure of head eversion. Various mutant combinations produce specific patterns of ectopic wing vein formation or melanotic scabs on the cuticle. Anaphase chromosome bridging is also seen as a maternal effect during the early embryonic nuclear divisions. In addition, specific behavioral defects such as poor climbing and flightlessness are detected among these mutants. Comparisons with other Drosophila mutant phenotypes suggests potential CAM targets that may mediate these developmental and behavioral effects, and analysis of the CAM crystal structure suggests the structural consequences of the individual mutations.
ISSN:0016-6731
1943-2631
1943-2631
DOI:10.1093/genetics/147.4.1783