Apparent trans control of murine beta-glucuronidase synthesis by a temporal genetic element

A difference in the heat-inactivation kinetics between the beta-glucuronidases of C3HeB/FeJ and C57B1/6J mice was utilized to assess the mode of action of a temporal genetic element in controlling the expression of the beta-glucuronidase structural gene Gus. The heat-inactivation kinetics of liver a...

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Bibliographic Details
Published in:Genetics (Austin) 1978-12, Vol.90 (4), p.725-734
Main Authors: Meredith, S A, Ganschow, R E
Format: Article
Language:English
Subjects:
Alleles
Animals
Genes
Glucuronidase - genetics
Glucuronidase - metabolism
History of medicine
History, 18th Century
Investigations
Kinetics
Mice
Mice, Inbred C3H
Mice, Inbred C57BL
Species Specificity
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Summary:A difference in the heat-inactivation kinetics between the beta-glucuronidases of C3HeB/FeJ and C57B1/6J mice was utilized to assess the mode of action of a temporal genetic element in controlling the expression of the beta-glucuronidase structural gene Gus. The heat-inactivation kinetics of liver and kidney beta-glucuronidase from F1 C3HeB/FeJ X C57B1/6J animals were intermediate with respect to the parental enzyme patterns, suggesting that equal concentrations of the two allelic products were present in beta-glucuronidase tetramers of F1 progeny. beta-glucuronidase heteropolymers assembled in vivo under conditions where equal concentrations of the two structural alleles of the enzyme were known to be present also exhibited intermediate heat-inactivation kinetics. These observations are consistent with a trans mode of action of a genetic element that controls the rate of murine beta-glucuronidase synthesis.
ISSN:0016-6731
1943-2631
1943-2631
DOI:10.1093/genetics/90.4.725