Agents that cause a high frequency of genetic change from [psi+] to [psi-] in Saccharomyces cerevisiae

The [psi] factor of yeast is cytoplasmically inherited. Singh, Helms and Sherman (1979) reported that high concentrations of KCl and of ethylene glycol induce the genetic change from [psi+] to [psi-]. In this study, the following agents have been shown to induce the same genetic change: guanidine hy...

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Bibliographic Details
Published in:Genetics (Austin) 1981-08, Vol.98 (4), p.691-711
Main Authors: Tuite, M.F, Mundy, C.R, Cox, B.S
Format: Article
Language:English
Subjects:
Dimethyl Sulfoxide - pharmacology
DNA, Fungal - genetics
DNA, Mitochondrial - genetics
Ethanol - pharmacology
Guanidines - pharmacology
Investigations
Methanol - pharmacology
Mutagens
plant breeding
plant genetics
Saccharomyces cerevisiae
Saccharomyces cerevisiae - genetics
Suppression, Genetic
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Summary:The [psi] factor of yeast is cytoplasmically inherited. Singh, Helms and Sherman (1979) reported that high concentrations of KCl and of ethylene glycol induce the genetic change from [psi+] to [psi-]. In this study, the following agents have been shown to induce the same genetic change: guanidine hydrochloride at 1 mM, dimethyl sulfoxide at 2.5% v/v and ethanol or methanol at 10% v/v. It is likely that a number of other agents also cause the change, namely 2 M glycerol, M succinate, M glutamate and M MgCl2. Most of these agents induce the change at very high frequencies; with some, the frequency is 100%. Although the observed phenotypic change can also occur as a result of chromosomal gene mutation, no changes of this type were identified. Some of the agents also cause mutation from [rho+] to [rho-] and from killer to sensitive.
ISSN:0016-6731
1943-2631
1943-2631
DOI:10.1093/genetics/98.4.691