Cloning and expression analysis of murine phospholipase D1

Activation of phosphatidylcholine-specific phospholipase D(PLD) occurs as part of the complex signal-transduction cascade initiated by agonist stimulation of tyrosine kinase and G-protein-coupled receptors. A variety of mammalian PLD activities have been described, and cDNAs for two PLDs recently re...

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Bibliographic Details
Published in:Biochemical journal 1997-09, Vol.326 ( Pt 3) (3), p.745-753
Main Authors: Colley, W C, Altshuller, Y M, Sue-Ling, C K, Copeland, N G, Gilbert, D J, Jenkins, N A, Branch, K D, Tsirka, S E, Bollag, R J, Bollag, W B, Frohman, M A
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Base Sequence
Chromosome Mapping
Cloning, Molecular
Gene Expression
In Situ Hybridization
Mice
Molecular Sequence Data
Organ Specificity
Phospholipase D - genetics
Phospholipase D - metabolism
Sequence Alignment
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Summary:Activation of phosphatidylcholine-specific phospholipase D(PLD) occurs as part of the complex signal-transduction cascade initiated by agonist stimulation of tyrosine kinase and G-protein-coupled receptors. A variety of mammalian PLD activities have been described, and cDNAs for two PLDs recently reported (human PLD1 and murine PLD2). We describe here the cloning and chromosomal localization of murine PLD1. Northern-blot hybridization and RNase protection analyses were used to examine the expression of murine PLD1 and PLD2 ina variety of cell lines and tissues. PLD1 and PLD2 were expressed in all RNA samples examined, although the absolute expression of each isoform varied, as well as the ratio of PLD1 to PLD2. Moreover, in situ hybridization of adult brain and murine embryo sections revealed high levels of expression of individual PLDs in some cell types and no detectable expression in others. Thus the two PLDs probably carry out distinct roles in restricted subsets of cells rather than ubiquitous roles in all cells.
ISSN:0264-6021
1470-8728
DOI:10.1042/bj3260745