Differential regulation by a peroxisome proliferator of the different multifunctional proteins in guinea pig: cDNA cloning of the guinea pig D-specific multifunctional protein 2

• Published in: Biochemical journal 1998-03, Vol.330 ( Pt 3) (3), p.1361-1368
• Main Authors: Caira, F, Clémencet, M C, Cherkaoui-Malki, M, Dieuaide-Noubhani, M, Pacot, C, Van Veldhoven, P P, Latruffe, N
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Animals; Base Sequence; Clofibric Acid - analogs & derivatives; Clofibric Acid - pharmacology; Cloning, Molecular; DNA, Complementary; Enoyl-CoA Hydratase - metabolism; Estradiol Dehydrogenases - biosynthesis; Estradiol Dehydrogenases - chemistry; Fibric Acids; Gene Expression Regulation - drug effects; Guinea Pigs; Hypolipidemic Agents - pharmacology; Liver - drug effects; Liver - metabolism; Male; Microbodies - drug effects; Microbodies - enzymology; Microbodies - physiology; Molecular Sequence Data; Oxidoreductases - metabolism; Rats; Rats, Sprague-Dawley; RNA, Messenger - biosynthesis; Transcription, Genetic - drug effects
• ISSN: 0264-6021; 1470-8728
• DOI: 10.1042/bj3301361

After our previous report on the cloning of two cDNA species in guinea pig, both encoding the same hepatic 79 kDa multifunctional protein 1 (MFP-1) [Caira, Cherkaoui-Malki, Hoefler and Latruffe (1996) FEBS Lett. 378, 57-60], here we report the cloning of a cDNA encoding a second multifunctional peroxisomal protein (MFP-2) in guinea-pig liver. This 2356 nt cDNA encodes a protein of 735 residues (79.7 kDa) whose sequence shows 83% identity with rat MFP-2 [Dieuaide-Noubhani, Novikov, Baumgart, Vanhooren, Fransen, Goethals, Vandekerckhove, Van Veldhoven and Mannaerts (1996) Eur. J. Biochem. 240, 660-666]. In parallel, we studied the effect of ciprofibrate, a hypolipaemic agent also known as peroxisome proliferator in rodent, on the expression of MFP-1 and MFP-2 (2.6 kb) in rats and guinea pigs. By Northern blotting analysis we demonstrated that three MFP-1-related mRNA species are expressed in the guinea-pig liver. The expression of two of them (3.5 and 2.6 kb) is slightly increased by ciprofibrate, whereas the 3.0 kb MFP-1 mRNA is, unlike the rat one, strongly down-regulated in guinea pigs treated with ciprofibrate. In a similar way, the hepatic expression of the guinea-pig 2.6 kb MFP-2 mRNA is also down-regulated in guinea pigs treated with ciprofibrate. These results demonstrate (1) that in contrast with the unique 3.0 kb MFP-1 rat mRNA, at least three hepatic MFP-1-related mRNA species are co-expressed in guinea pig; and (2) that, opposed to the accepted idea of non-responsiveness of the guinea pig to ciprofibrate, this drug affects MFP-1 and MFP-2 gene expression in this species. Also, the mRNA species for acyl-CoA oxidase and thiolase, two other enzymes of the peroxisomal beta-oxidation pathway that are induced severalfold in responsive species are down-regulated in guinea pig. This paper is the first, to our knowledge, reporting the down-regulation of the expression of genes encoding enzymes involved in the peroxisomal beta-oxidation of fatty acids (MFP-1) and bile acid synthesis (MFP-2) in mammals.