Activity of the human cytochrome c1 promoter is modulated by E2F

The human cytochrome c(1) promoter is strongly activated in transfected Drosophila SL2 cells expressing exogenous human E2F1. Transfection-deletion experiments, DNase I protection by E2F1 and gel mobility-shift experiments locate E2F1 activation sites to two regions on either side of the transcripti...

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Bibliographic Details
Published in:Biochemical journal 2000-10, Vol.351 (Pt 1), p.251-256
Main Authors: Luciakova, K, Barath, P, Li, R, Zaid, A, Nelson, B D
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Binding Sites
Carrier Proteins
Cell Cycle Proteins
Cell Line
Cell Nucleus - genetics
Cytochromes c1 - genetics
Deoxyribonuclease I - metabolism
DNA - genetics
DNA - metabolism
DNA Footprinting
DNA-Binding Proteins - chemistry
DNA-Binding Proteins - genetics
DNA-Binding Proteins - metabolism
Drosophila melanogaster
Drosophila Proteins
E2F Transcription Factors
E2F1 Transcription Factor
E2F6 Transcription Factor
Gene Expression Regulation
HeLa Cells
Humans
Mitochondria - metabolism
Oxidative Phosphorylation
Promoter Regions, Genetic - genetics
Protein Structure, Tertiary
Repressor Proteins - chemistry
Repressor Proteins - genetics
Repressor Proteins - metabolism
Response Elements - genetics
Retinoblastoma-Binding Protein 1
Sequence Deletion - genetics
Substrate Specificity
Trans-Activators
Transcription Factor DP1
Transcription Factors - chemistry
Transcription Factors - genetics
Transcription Factors - metabolism
Transfection
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Summary:The human cytochrome c(1) promoter is strongly activated in transfected Drosophila SL2 cells expressing exogenous human E2F1. Transfection-deletion experiments, DNase I protection by E2F1 and gel mobility-shift experiments locate E2F1 activation sites to two regions on either side of the transcription start site. Deletion of either region prevents E2F1 activation in transfected SL2 cells, suggesting a co-operative interaction between them. E2F6, a member of the E2F family that lacks transactivation domains but contains specific suppressor domains, inhibits cytochrome c(1) promoter activity when co-transfected into HeLa cells, indicating that the E2F proteins modulate the cytochrome c(1) promoter in mammalian cells. However, E2F is not a general regulator of oxidative phosphorylation genes since three additional nuclear-encoded mitochondrial genes were unaffected by E2F1 or E2F6.
ISSN:0264-6021
1470-8728
DOI:10.1042/0264-6021:3510251