Gated conductances in native and reconstituted membranes from frog olfactory cilia

Although cAMP is well established as a second messenger for olfactory transduction in vertebrates, the role of inositol 1,4,5-trisphosphate (IP3) in this process remains controversial. We addressed this issue by comparing currents evoked by cAMP and IP3 in native and reconstituted membranes from olf...

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Bibliographic Details
Published in:Biophysical journal 1996-02, Vol.70 (2), p.813-817
Main Authors: Nakamura, T., Lee, H.H., Kobayashi, H., Satoh, T.O.
Format: Article
Language:English
Subjects:
Animals
Biophysical Phenomena
Biophysics
Cilia - drug effects
Cilia - metabolism
Cyclic AMP - pharmacology
Electric Conductivity
In Vitro Techniques
Inositol 1,4,5-Trisphosphate - pharmacology
Ion Channel Gating - drug effects
Ion Channels - drug effects
Ion Channels - metabolism
Lipid Bilayers - metabolism
Membranes, Artificial
Olfactory Mucosa - drug effects
Olfactory Mucosa - metabolism
Rana catesbeiana
Second Messenger Systems
Signal Transduction
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Summary:Although cAMP is well established as a second messenger for olfactory transduction in vertebrates, the role of inositol 1,4,5-trisphosphate (IP3) in this process remains controversial. We addressed this issue by comparing currents evoked by cAMP and IP3 in native and reconstituted membranes from olfactory cilia. We detected only a cyclic nucleotide-gated conductance in the native membrane but both cyclic nucleotide-gated and IP3-gated conductances in the reconstituted membrane. The magnitudes of the cyclic nucleotide- and IP3-gated conductances were not correlated with each other in reconstituted membranes, suggesting that cyclic nucleotide- and IP3-gated channels originate in different cellular compartments.
ISSN:0006-3495
1542-0086
DOI:10.1016/S0006-3495(96)79625-X