Rapid Identification of Candida Species and Other Clinically Important Yeast Species by Flow Cytometry

Two rapid diagnostic assays, utilizing two different Luminex flow cytometry methods, were developed for identification of clinically important ascomycetous yeast species. Direct hybridization and allele-specific primer extension methods were both successful in establishing a DNA-based assay that can...

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Bibliographic Details
Published in:Journal of Clinical Microbiology 2005-09, Vol.43 (9), p.4507-4514
Main Authors: Page, Brent T, Kurtzman, Cletus P
Format: Article
Language:English
Subjects:
allele-specific primer extension assay
analysis
assays
Biological and medical sciences
Candida
Candida - classification
Candida - genetics
Candida albicans
Candida glabrata
Candida krusei
Candida parapsilosis
Candida tropicalis
Candidiasis
Candidiasis - microbiology
classification
direct hybridization assay
DNA primers
DNA Probes
DNA, Fungal
DNA, Fungal - analysis
flow cytometry
Flow Cytometry - methods
Fundamental and applied biological sciences. Psychology
genetics
Humans
Infectious diseases
Medical sciences
methods
Microbiology
Miscellaneous
Mycological Typing Techniques
Mycology
Mycoses
Mycoses - microbiology
nucleic acid hybridization
Nucleic Acid Hybridization - methods
oligonucleotide probes
pathogen identification
rapid methods
Species Specificity
strains
taxonomy
Time Factors
yeasts
Yeasts - classification
Yeasts - genetics
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Summary:Two rapid diagnostic assays, utilizing two different Luminex flow cytometry methods, were developed for identification of clinically important ascomycetous yeast species. Direct hybridization and allele-specific primer extension methods were both successful in establishing a DNA-based assay that can rapidly and accurately identify Candida albicans, Candida krusei, Candida parapsilosis, Candida glabrata, and Candida tropicalis as well as other clinical species. The direct hybridization assay was designed to identify a total of 19 ascomycetous yeast species, and the allele-specific primer extension assay was designed to identify a total of 34 species. Probes were validated against 438 strains representing 303 species. From culture to identification, the allele-specific primer extension method takes 8 h and the direct hybridization method takes less than 5 h to complete. These assays represent comprehensive, rapid tests that are well suited for the clinical laboratory.
ISSN:0095-1137
1098-660X
DOI:10.1128/JCM.43.9.4507-4514.2005