The exosome of Trypanosoma brucei

The yeast exosome is a complex of at least 10 essential 3′–5′ riboexonucleases which is involved in 3′‐processing of many RNA species. An exosome‐like complex has been found or predicted to exist in other eukaryotes but not in Escherichia coli . The unicellular parasite Trypanosoma brucei diverged v...

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Bibliographic Details
Published in:The EMBO journal 2001-07, Vol.20 (14), p.3831-3839
Main Authors: Estévez, Antonio M., Kempf, Tore, Clayton, Christine
Format: Article
Language:English
Subjects:
Alleles
Amino Acid Sequence
Animals
Base Sequence
Cloning, Molecular
DNA Primers
E coli
exoribonucleases
Exoribonucleases - chemistry
Exoribonucleases - metabolism
exosome
exosomes
Genetic Complementation Test
Mass Spectrometry
Molecular Sequence Data
Parasites
ribonuclease
RNA, Ribosomal, 5.8S - genetics
RNA, Ribosomal, 5.8S - metabolism
rRNA 5.8S
rRNA processing
Saccharomyces cerevisiae - genetics
Trypanosoma brucei
Trypanosoma brucei brucei - enzymology
Yeasts
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Summary:The yeast exosome is a complex of at least 10 essential 3′–5′ riboexonucleases which is involved in 3′‐processing of many RNA species. An exosome‐like complex has been found or predicted to exist in other eukaryotes but not in Escherichia coli . The unicellular parasite Trypanosoma brucei diverged very early in eukaryotic evolution. We show here that T.brucei contains at least eight exosome subunit homologs, but only a subset of these associate in a complex. Accordingly, the T.brucei exosome is smaller than that of yeast. Both free and complex‐associated homologs are essential for cell viability and are involved in 5.8S rRNA maturation. We suggest that the exosome was present in primitive eukaryotes, and became increasingly complex during subsequent evolution.
ISSN:0261-4189
1460-2075
1460-2075
DOI:10.1093/emboj/20.14.3831