Levels of circumsporozoite protein in the Plasmodium oocyst determine sporozoite morphology

The sporozoite stage of the Plasmodium parasite is formed by budding from a multinucleate oocyst in the mosquito midgut. During their life, sporozoites must infect the salivary glands of the mosquito vector and the liver of the mammalian host; both events depend on the major sporozoite surface prote...

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Published in:The EMBO journal 2002-04, Vol.21 (7), p.1586-1596
Main Authors: Thathy, Vandana, Fujioka, Hisashi, Gantt, Soren, Nussenzweig, Ruth, Nussenzweig, Victor, Ménard, Robert
Format: Article
Language:English
Subjects:
ACS protein
Animals
Anopheles
Aquatic insects
budding
cell division
circumsporozoite protein
circumsporozoites
EMBO05
EMBO23
Humans
Microtubules - metabolism
Morphogenesis
Mosquitoes
Mutagenesis
Oocysts
Parasites
Phenotype
Plasmodium berghei
Plasmodium berghei - genetics
Plasmodium berghei - metabolism
Plasmodium berghei - ultrastructure
Protozoan Proteins - biosynthesis
Protozoan Proteins - genetics
Protozoan Proteins - metabolism
Rats
Rats, Sprague-Dawley
sporozoite
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container_issue 7
container_start_page 1586
container_title The EMBO journal
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creator Thathy, Vandana
Fujioka, Hisashi
Gantt, Soren
Nussenzweig, Ruth
Nussenzweig, Victor
Ménard, Robert
description The sporozoite stage of the Plasmodium parasite is formed by budding from a multinucleate oocyst in the mosquito midgut. During their life, sporozoites must infect the salivary glands of the mosquito vector and the liver of the mammalian host; both events depend on the major sporozoite surface protein, the circumsporozoite protein (CS). We previously reported that Plasmodium berghei oocysts in which the CS gene is inactivated do not form sporozoites. Here, we analyzed the ultrastructure of P.berghei oocyst differentiation in the wild type, recombinants that do not produce or produce reduced amounts of CS, and corresponding complemented clones. The results indicate that CS is essential for establishing polarity in the oocyst. The amounts of CS protein correlate with the extent of development of the inner membranes and associated microtubules underneath the oocyst outer membrane, which normally demarcate focal budding sites. This is a first example of a protein controlling both morphogenesis and infectivity of a parasite stage.
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identifier ISSN: 0261-4189
ispartof The EMBO journal, 2002-04, Vol.21 (7), p.1586-1596
issn 0261-4189
1460-2075
1460-2075
language eng
recordid cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_125957
source PubMed Central
subjects ACS protein
Animals
Anopheles
Aquatic insects
budding
cell division
circumsporozoite protein
circumsporozoites
EMBO05
EMBO23
Humans
Microtubules - metabolism
Morphogenesis
Mosquitoes
Mutagenesis
Oocysts
Parasites
Phenotype
Plasmodium berghei
Plasmodium berghei - genetics
Plasmodium berghei - metabolism
Plasmodium berghei - ultrastructure
Protozoan Proteins - biosynthesis
Protozoan Proteins - genetics
Protozoan Proteins - metabolism
Rats
Rats, Sprague-Dawley
sporozoite
title Levels of circumsporozoite protein in the Plasmodium oocyst determine sporozoite morphology
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