Cryo-EM of the native structure of the calcium release channel/ryanodine receptor from sarcoplasmic reticulum

The native structure of the calcium release channel (ryanodine receptor) from rabbit skeletal muscle has been analyzed in two dimensions from electron micrographs of frozen hydrated specimens. Within a resolution of 3.0 nm there is excellent agreement between the structure as seen in vitreous water...

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Bibliographic Details
Published in:Biophysical journal 1992-04, Vol.61 (4), p.936-940
Main Authors: Radermacher, M., Wagenknecht, T., Grassucci, R., Frank, J., Inui, M., Chadwick, C., Fleischer, S.
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Animals
Binding and carrier proteins
Biological and medical sciences
Biophysical Phenomena
Biophysics
Calcium Channels - ultrastructure
Freezing
Fundamental and applied biological sciences. Psychology
Microscopy, Electron
Muscle Proteins - ultrastructure
Protein Conformation
Proteins
Rabbits
Receptors, Cholinergic - ultrastructure
Ryanodine Receptor Calcium Release Channel
Sarcoplasmic Reticulum - ultrastructure
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Summary:The native structure of the calcium release channel (ryanodine receptor) from rabbit skeletal muscle has been analyzed in two dimensions from electron micrographs of frozen hydrated specimens. Within a resolution of 3.0 nm there is excellent agreement between the structure as seen in vitreous water and in negative stained specimens. Features seen in the three-dimensional reconstruction of the negatively stained channel can be identified in the projection of the unstained receptor.
ISSN:0006-3495
1542-0086
DOI:10.1016/S0006-3495(92)81900-8