pathogenesis study of foot-and-mouth disease in cattle, using in situ hybridization

Eight calves were exposed in an aerosol chamber to nebulized foot-and-mouth disease virus. Two control animals were exposed in a similar manner to cell culture media only. Animals were euthanized at intervals and various tissues examined by in situ hybridization using a biotinylated RNA probe corres...

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Bibliographic Details
Published in:Canadian journal of veterinary research 1992-07, Vol.56 (3), p.189-193
Main Authors: Brown, C.C, Meyer, R.F, Olander, H.J, House, C, Mebus, C.A
Format: Article
Language:English
Subjects:
Animals
Aphthovirus
Aphthovirus - genetics
Aphthovirus - isolation & purification
Aphthovirus - physiology
calves
Carpus, Animal - microbiology
Cattle
Cattle Diseases - microbiology
Cattle Diseases - pathology
disease course
experimental infections
Eyelids - microbiology
foot-and-mouth disease
Foot-and-Mouth Disease - microbiology
Foot-and-Mouth Disease - pathology
Hoof and Claw - microbiology
Hoof and Claw - pathology
Lung - microbiology
Lung - pathology
Nucleic Acid Hybridization
Palatine Tonsil - pathology
pathogenesis
RNA Probes
RNA, Viral - analysis
Tongue - microbiology
Tongue - pathology
Viremia - microbiology
Viremia - veterinary
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Summary:Eight calves were exposed in an aerosol chamber to nebulized foot-and-mouth disease virus. Two control animals were exposed in a similar manner to cell culture media only. Animals were euthanized at intervals and various tissues examined by in situ hybridization using a biotinylated RNA probe corresponding to a portion of the viral gene coding for the polymerase enzyme. By this technique large amounts of viral nucleic acid were found in coronary band, interdigital cleft and tongue as early as six hours postexposure, indicating a very rapid delivery from the portal of entry to the predilection sites for lesion development. This occurred well before the onset of viremia which by virus isolation was not detectable until 30 hours postexposure. The in situ hybridization signal in these tissues decreased in intensity and extent with time to focally positive areas, occasionally surrounding a vesicle. Other epidermal sites not normally thought of as sites for foot-and-mouth lesion development, such as carpus and eyelid, also had some viral nucleic acid detectable at various time intervals. In the lung by in situ hybridization, alveolar septa had viral nucleic acid early in infection (6-18 h postexposure) while later (36-96 h postexposure), the in situ hybridization signal was prominent in alveolar macrophages.
ISSN:0830-9000