The subcellular distribution and properties of hexokinases in the guinea-pig cerebral cortex

1. Hexokinase activities were estimated in primary subcellular fractions from guinea-pig cerebral cortex and in sucrose-density-gradient subfractions of the mitochondrial and microsomal fractions. 2. Appreciable activities were observed in mitochondrial, microsomal and soluble fractions. The activit...

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Bibliographic Details
Published in:Biochemical journal 1967-07, Vol.104 (1), p.286-292
Main Author: Bachelard, H S
Format: Article
Language:English
Subjects:
Adenosine Triphosphate - metabolism
Animals
Centrifugation, Density Gradient
Cerebral Cortex - cytology
Cerebral Cortex - enzymology
Electrophoresis
Glucose - metabolism
Guinea Pigs
Hexokinase - metabolism
In Vitro Techniques
Kinetics
Microsomes - enzymology
Mitochondria - enzymology
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Summary:1. Hexokinase activities were estimated in primary subcellular fractions from guinea-pig cerebral cortex and in sucrose-density-gradient subfractions of the mitochondrial and microsomal fractions. 2. Appreciable activities were observed in mitochondrial, microsomal and soluble fractions. The activity in the mitochondrial fraction was associated with the mitochondria rather than with myelin or nerve endings and that in the microsomal fraction was associated with membrane fragments. 3. Most of the mitochondrial activity was extracted in soluble form by osmotic ;shock'. The activity of the mitochondrial extract differed from the soluble activity in kinetic properties and in electrophoretic behaviour. 4. No evidence was obtained for the presence of a high-K(m) glucokinase in the brain. 5. The results are discussed in terms of relevance to considerations of glucose utilization by the brain.
ISSN:0264-6021
1470-8728
DOI:10.1042/bj1040286