The calcium-stimulated incorporation of ethanolamine and serine into the phospholipids of the housefly Musca domestica

1. The calcium-stimulated incorporation of [2-(14)C]ethanolamine and l-[3-(14)C]-serine into the phospholipids of homogenates of the fat bodies from larval houseflies (Musca domestica) was studied. 2. Ethanolamine and serine acted as competitive inhibitors with one another. N-Methylethanolamine was...

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Published in:Biochemical journal 1967-08, Vol.104 (2), p.695-704
Main Author: Crone, H D
Format: Article
Language:English
Subjects:
Amino Alcohols - metabolism
Animals
Calcium - pharmacology
Carbon Isotopes
Houseflies - drug effects
Hydrogen-Ion Concentration
Metals - pharmacology
Phospholipases - metabolism
Phospholipids - metabolism
Phosphorus Isotopes
Serine - metabolism
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description 1. The calcium-stimulated incorporation of [2-(14)C]ethanolamine and l-[3-(14)C]-serine into the phospholipids of homogenates of the fat bodies from larval houseflies (Musca domestica) was studied. 2. Ethanolamine and serine acted as competitive inhibitors with one another. N-Methylethanolamine was not distinguished from ethanolamine by the system. Tris buffer was also a competitor with these compounds, and a number of other amino alcohols were inhibitory, probably competitively. 3. The incorporation of [(32)P]phosphorylethanolamine into phospholipids was observed in suspensions of whole fat bodies. This incorporation was stimulated by magnesium. 4. During the incubation of the homogenates, a calcium-stimulated breakdown of phospholipids by a phospholipase A occurred. 5. These results are compared with results published for similar mammalian systems, and their possible physiological significance is discussed.
doi_str_mv 10.1042/bj1040695
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The calcium-stimulated incorporation of [2-(14)C]ethanolamine and l-[3-(14)C]-serine into the phospholipids of homogenates of the fat bodies from larval houseflies (Musca domestica) was studied. 2. Ethanolamine and serine acted as competitive inhibitors with one another. N-Methylethanolamine was not distinguished from ethanolamine by the system. Tris buffer was also a competitor with these compounds, and a number of other amino alcohols were inhibitory, probably competitively. 3. The incorporation of [(32)P]phosphorylethanolamine into phospholipids was observed in suspensions of whole fat bodies. This incorporation was stimulated by magnesium. 4. During the incubation of the homogenates, a calcium-stimulated breakdown of phospholipids by a phospholipase A occurred. 5. 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The calcium-stimulated incorporation of [2-(14)C]ethanolamine and l-[3-(14)C]-serine into the phospholipids of homogenates of the fat bodies from larval houseflies (Musca domestica) was studied. 2. Ethanolamine and serine acted as competitive inhibitors with one another. N-Methylethanolamine was not distinguished from ethanolamine by the system. Tris buffer was also a competitor with these compounds, and a number of other amino alcohols were inhibitory, probably competitively. 3. The incorporation of [(32)P]phosphorylethanolamine into phospholipids was observed in suspensions of whole fat bodies. This incorporation was stimulated by magnesium. 4. During the incubation of the homogenates, a calcium-stimulated breakdown of phospholipids by a phospholipase A occurred. 5. 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The calcium-stimulated incorporation of [2-(14)C]ethanolamine and l-[3-(14)C]-serine into the phospholipids of homogenates of the fat bodies from larval houseflies (Musca domestica) was studied. 2. Ethanolamine and serine acted as competitive inhibitors with one another. N-Methylethanolamine was not distinguished from ethanolamine by the system. Tris buffer was also a competitor with these compounds, and a number of other amino alcohols were inhibitory, probably competitively. 3. The incorporation of [(32)P]phosphorylethanolamine into phospholipids was observed in suspensions of whole fat bodies. This incorporation was stimulated by magnesium. 4. During the incubation of the homogenates, a calcium-stimulated breakdown of phospholipids by a phospholipase A occurred. 5. 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subjects Amino Alcohols - metabolism
Animals
Calcium - pharmacology
Carbon Isotopes
Houseflies - drug effects
Hydrogen-Ion Concentration
Metals - pharmacology
Phospholipases - metabolism
Phospholipids - metabolism
Phosphorus Isotopes
Serine - metabolism
title The calcium-stimulated incorporation of ethanolamine and serine into the phospholipids of the housefly Musca domestica
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