Ca2+-Calmodulin-dependent Protein Kinase II Potentiates Store-operated Ca2+ Current

A rise in intracellular Ca2+ (Ca2+i) mediates various cellular functions ranging from fertilization to gene expression. A ubiquitous Ca2+ influx pathway that contributes significantly to the generation of Ca2+i signals, especially in non-excitable cells, is store-operated Ca2+ entry (SOCE). Conseque...

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Bibliographic Details
Published in:The Journal of biological chemistry 2003-09, Vol.278 (36), p.33730-33737
Main Author: Machaca, Khaled
Format: Article
Language:English
Subjects:
Animals
Biological Transport
Calcium - metabolism
Calcium Channels - metabolism
Calcium-Calmodulin-Dependent Protein Kinase Type 2
Calcium-Calmodulin-Dependent Protein Kinases - metabolism
Calcium-Calmodulin-Dependent Protein Kinases - physiology
Electrophysiology
Ion Channel Gating
Kinetics
Microscopy, Confocal
Oocytes - metabolism
Phosphorylation
Receptors, Serotonin - metabolism
Time Factors
Xenopus laevis
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Summary:A rise in intracellular Ca2+ (Ca2+i) mediates various cellular functions ranging from fertilization to gene expression. A ubiquitous Ca2+ influx pathway that contributes significantly to the generation of Ca2+i signals, especially in non-excitable cells, is store-operated Ca2+ entry (SOCE). Consequently, the modulation of SOCE current affects Ca2+i dynamics and thus the ensuing cellular response. Therefore, it is important to define the mechanisms that regulate SOCE. Here we show that a rise in Ca2+i potentiates SOCE. This potentiation is mediated by Ca2+-calmodulin-dependent protein kinase II (CaMKII), because inhibition of endogenous CaMKII activity abrogates Ca2+i -mediated SOCE potentiation and expression of constitutively active CaMKII potentiates SOCE current independently of Ca2+i. Moreover, we present evidence that CaMKII potentiates SOCE by altering SOCE channel gating. The regulation of SOCE by CaMKII defines a novel modulatory mechanism of SOCE with important physiological consequences.
ISSN:0021-9258
1083-351X
DOI:10.1074/jbc.M305023200