UXT is a novel centrosomal protein essential for cell viability

Ubiquitously expressed transcript (UXT) is a prefoldinlike protein that has been suggested to be involved in human tumorigenesis. Here, we have found that UXT is overexpressed in a number of human tumor tissues but not in the matching normal tissues. We demonstrate that UXT is located in human centr...

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Bibliographic Details
Published in:Molecular biology of the cell 2005-12, Vol.16 (12), p.5857-5865
Main Authors: Zhao, Huiwu, Wang, Qiang, Zhang, Hongtao, Liu, Qingdu, Du, Xiulian, Richter, Mark, Greene, Mark I
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Bone Neoplasms
Cell Line, Tumor
Cell Survival - physiology
Centrosome - physiology
Centrosome - ultrastructure
Genetic Vectors
Humans
Microscopy, Electron
Molecular Sequence Data
Mutagenesis
Neoplasm Proteins - chemistry
Neoplasm Proteins - genetics
Neoplasm Proteins - physiology
Osteosarcoma
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Summary:Ubiquitously expressed transcript (UXT) is a prefoldinlike protein that has been suggested to be involved in human tumorigenesis. Here, we have found that UXT is overexpressed in a number of human tumor tissues but not in the matching normal tissues. We demonstrate that UXT is located in human centrosomes and is associated with gamma-tubulin. In addition, overexpression of UXT disrupts centrosome structure. Furthermore, abrogation of UXT protein expression by small interfering RNA knockdown leads to cell death. Together, our findings suggest that UXT is a component of centrosome and is essential for cell viability. We propose that UXT may facilitate transformation by corrupting regulated centrosome functions.
ISSN:1059-1524
1939-4586
DOI:10.1091/mbc.e05-08-0705