In control: systematic assessment of microarray performance

Expression profiling using DNA microarrays is a powerful technique that is widely used in the life sciences. How reliable are microarray-derived measurements? The assessment of performance is challenging because of the complicated nature of microarray experiments and the many different technology pl...

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Bibliographic Details
Published in:EMBO reports 2004-10, Vol.5 (10), p.964-969
Main Authors: van Bakel, Harm, Holstege, Frank C P
Format: Article
Language:English
Subjects:
Animals
DNA, Complementary - analysis
expression profiling
external controls
Gene Expression Profiling
Humans
microarray
Oligonucleotide Array Sequence Analysis
Performance assessment
quality
Review
RNA - analysis
Spectrophotometry
spikes
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Summary:Expression profiling using DNA microarrays is a powerful technique that is widely used in the life sciences. How reliable are microarray-derived measurements? The assessment of performance is challenging because of the complicated nature of microarray experiments and the many different technology platforms. There is a mounting call for standards to be introduced, and this review addresses some of the issues that are involved. Two important characteristics of performance are accuracy and precision. The assessment of these factors can be either for the purpose of technology optimization or for the evaluation of individual microarray hybridizations. Microarray performance has been evaluated by at least four approaches in the past. Here, we argue that external RNA controls offer the most versatile system for determining performance and describe how such standards could be implemented. Other uses of external controls are discussed, along with the importance of probe sequence availability and the quantification of labelled material.
ISSN:1469-221X
1469-3178
1469-221X
DOI:10.1038/sj.embor.7400253