Localized Dynamic Light Scattering: A New Approach to Dynamic Measurements in Optical Microscopy

We present a new approach to probing single-particle dynamics that uses dynamic light scattering from a localized region. By scattering a focused laser beam from a micron-size particle, we measure its spatial fluctuations via the temporal autocorrelation of the scattered intensity. We demonstrate th...

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Bibliographic Details
Published in:Biophysical journal 1998-03, Vol.74 (3), p.1541-1548
Main Authors: Meller, A., Bar-Ziv, R., Tlusty, T., Moses, E., Stavans, J., Safran, S.A.
Format: Article
Language:English
Subjects:
Biophysics - methods
Lasers
Light
Micromanipulation - methods
Microscopy - methods
Models, Theoretical
Scattering, Radiation
Silicon Dioxide
Time Factors
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Summary:We present a new approach to probing single-particle dynamics that uses dynamic light scattering from a localized region. By scattering a focused laser beam from a micron-size particle, we measure its spatial fluctuations via the temporal autocorrelation of the scattered intensity. We demonstrate the applicability of this approach by measuring the three-dimensional force constants of a single bead and a pair of beads trapped by laser tweezers. The scattering equations that relate the scattered intensity autocorrelation to the particle position correlation function are derived. This technique has potential applications for measurement of biomolecular force constants and probing viscoelastic properties of complex media.
ISSN:0006-3495
1542-0086
DOI:10.1016/S0006-3495(98)77866-X