Interferon- γ and Sinusoidal Electric Fields Signal by Modulating NAD(P)H Oscillations in Polarized Neutrophils

Metabolic activity in eukaryotic cells is known to naturally oscillate. We have recently observed a 20-s period NAD(P)H oscillation in neutrophils and other polarized cells. Here we show that when polarized human neutrophils are exposed to interferon- γ or to ultra-low-frequency electric fields with...

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Bibliographic Details
Published in:Biophysical journal 2000-12, Vol.79 (6), p.3001-3008
Main Authors: Rosenspire, Allen J., Kindzelskii, Andrei L., Petty, Howard R.
Format: Article
Language:English
Subjects:
Cell Size
Electric Stimulation
Humans
In Vitro Techniques
Interferon-gamma - pharmacology
Kinetics
NAD - blood
NADP - blood
Neutrophils - cytology
Neutrophils - drug effects
Neutrophils - physiology
Oscillometry
Reactive Oxygen Species - metabolism
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Summary:Metabolic activity in eukaryotic cells is known to naturally oscillate. We have recently observed a 20-s period NAD(P)H oscillation in neutrophils and other polarized cells. Here we show that when polarized human neutrophils are exposed to interferon- γ or to ultra-low-frequency electric fields with periods double that of the NAD(P)H oscillation, the amplitude of the NAD(P)H oscillations increases. Furthermore, increases in NAD(P)H amplitude, whether mediated by interferon- γ or by an oscillating electric field, signals increased production of reactive oxygen metabolites. Hence, amplitude modulation of NAD(P)H oscillations suggests a novel signaling mechanism in polarized cells.
ISSN:0006-3495
1542-0086
DOI:10.1016/S0006-3495(00)76536-2