Optical Anisotropy in Lipid Bilayer Membranes: Coupled Plasmon-Waveguide Resonance Measurements of Molecular Orientation, Polarizability, and Shape

The birefringence and linear dichroism of anisotropic thin films such as proteolipid membranes are related to molecular properties such as polarizability, shape, and orientation. Coupled plasmon-waveguide resonance (CPWR) spectroscopy is shown in the present work to provide a convenient means of eva...

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Bibliographic Details
Published in:Biophysical journal 2001-03, Vol.80 (3), p.1557-1567
Main Authors: Salamon, Zdzislaw, Tollin, Gordon
Format: Article
Language:English
Subjects:
Anisotropy
Birefringence
Boron Compounds
Chemical reactions
Equipment Design
Fluorescent Dyes
Lipid Bilayers - chemistry
Lipids
Membranes
Molecules
Orientation
Phosphatidylcholines - chemistry
Proteins
Proteolipids - chemistry
Surface Plasmon Resonance - instrumentation
Surface Plasmon Resonance - methods
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Summary:The birefringence and linear dichroism of anisotropic thin films such as proteolipid membranes are related to molecular properties such as polarizability, shape, and orientation. Coupled plasmon-waveguide resonance (CPWR) spectroscopy is shown in the present work to provide a convenient means of evaluating these parameters in a single lipid bilayer. This is illustrated by using 1–10 mol % of an acyl chain chromophore-labeled phosphatidylcholine (PC) incorporated into a solid-supported PC bilayer deposited onto a hydrated silica surface. CPWR measurements were made of refractive index and extinction coefficient anisotropies with two exciting light wavelengths, one of which is absorbed by the chromophore and one of which is not. These results were used to calculate longitudinal and transverse molecular polarizabilities, the orientational order parameter and average angle between the longitudinal axis of the lipid molecule and the membrane normal, and the molecular shape factors of the lipid molecules. The values thereby obtained are in excellent agreement with parameters determined by other techniques, and provide a powerful tool for analyzing lipid-protein, protein-protein, and protein-ligand interactions in proteolipid films.
ISSN:0006-3495
1542-0086
DOI:10.1016/S0006-3495(01)76128-0