Effect of Contact Time and Force on Monocyte Adhesion to Vascular Endothelium

In this study we examined whether monocytic cell attachment to vascular endothelium was affected by elevating shear stress at a constant shear rate. Contact time, which is inversely related to the shear rate, was fixed and viscosity elevated with dextran to increase the shear stress (and hence the n...

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Bibliographic Details
Published in:Biophysical journal 2001-04, Vol.80 (4), p.1722-1732
Main Authors: Rinker, Kristina D., Prabhakar, Vikas, Truskey, George A.
Format: Article
Language:English
Subjects:
Biophysical Phenomena
Biophysics
Cell Adhesion
Cell Line
Cellular biology
Chemical bonds
Dextrans - pharmacology
Endothelium, Vascular - metabolism
Flow Cytometry
Humans
Kinetics
Models, Theoretical
Monocytes - cytology
Stress, Mechanical
Time
Time Factors
Umbilical Veins - cytology
Viscosity
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Summary:In this study we examined whether monocytic cell attachment to vascular endothelium was affected by elevating shear stress at a constant shear rate. Contact time, which is inversely related to the shear rate, was fixed and viscosity elevated with dextran to increase the shear stress (and hence the net force on the cell) independently of shear rate. At a fixed contact time, tethering frequencies increased, rolling velocities decreased, and median arrest durations increased with increasing shear stress. Rolling and short arrests (
ISSN:0006-3495
1542-0086
DOI:10.1016/S0006-3495(01)76143-7