A new method for labelling saxitoxin and its binding to non-myelinated fibres of the rabbit vagus, lobster walking leg, and garfish olfactory nerves

1. A new method of labelling saxitoxin (STX) is described, based on transfer of tritium from tritiated water to the toxin. 2. The radiochemical purity of the labelled toxin has been directly determined, rather than being based on indirect biochemical means, as in previous experiments with Wilzbach-l...

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Bibliographic Details
Published in:The Journal of physiology 1976-10, Vol.261 (2), p.477-494
Main Authors: Ritchie, J M, Rogart, R B, Strichartz, G R
Format: Article
Language:English
Subjects:
Animals
Binding Sites
Fishes
Homarus americanus
Isotope Labeling - methods
Muscles - innervation
Nephropidae
Nerve Fibers - metabolism
Olfactory Nerve - metabolism
Rabbits
Saxitoxin - metabolism
Sodium - metabolism
Tritium
Vagus Nerve - metabolism
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Summary:1. A new method of labelling saxitoxin (STX) is described, based on transfer of tritium from tritiated water to the toxin. 2. The radiochemical purity of the labelled toxin has been directly determined, rather than being based on indirect biochemical means, as in previous experiments with Wilzbach-labelled STX and TTX. 3. The specific activity of the labelled toxin, 66 d..m.f-mole-1, corresponds with one tritium atom per molecule STX, an improvement of about 300-fold over other means of labelling TTX and STX. 4. The binding of this toxin to rabbit, lobster and garfish olfactory nerve fibres has been re-examined. 5. The density of sodium channels calculated on the basis of the binding of the toxin is about four to six-times the values previously reported.
ISSN:0022-3751
1469-7793
DOI:10.1113/jphysiol.1976.sp011569