Analysis of nonexponential fluorescence decay data by a method of moments

A method of moments is presented for the analysis of convoluted fluorescence decay data when the impulse response function is given by f(t) = alpha exp (-At - Bt1/2). Examples of this method are given using both simulated and measured fluorescence decays. It is also shown that this method, used with...

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Bibliographic Details
Published in:Biophysical journal 1980-03, Vol.29 (3), p.367-378
Main Authors: Solie, T.N., Small, E.W., Isenberg, I.
Format: Article
Language:English
Subjects:
Benz(a)Anthracenes
Computers
Fluorescence
Fluorometry
Hydrocarbons, Brominated
Methods
Propylene Glycols
Time Factors
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Summary:A method of moments is presented for the analysis of convoluted fluorescence decay data when the impulse response function is given by f(t) = alpha exp (-At - Bt1/2). Examples of this method are given using both simulated and measured fluorescence decays. It is also shown that this method, used with moment index displacements, will correct for light-scatter leakage, zero-point time shifts, and slow lamp drift.
ISSN:0006-3495
1542-0086
DOI:10.1016/S0006-3495(80)85140-X