Fluorescence polarization spectroscopy and time-resolved fluorescence kinetics of native cancerous and normal rat kidney tissues

Steady state fluorescence polarization spectra and time-resolved emission decay kinetics have been measured in vitro from malignant and normal rat kidney tissue. The degrees of polarization and emission lifetimes from the cancerous and normal systems are different. The spectroscopic differences are...

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Bibliographic Details
Published in:Biophysical journal 1986-09, Vol.50 (3), p.463-469
Main Authors: Tata, D.B., Foresti, M., Cordero, J., Tomashefsky, P., Alfano, M.A., Alfano, R.R.
Format: Article
Language:English
Subjects:
Animals
Applied sciences
Biological and medical sciences
Exact sciences and technology
Flavins - analysis
Investigative techniques, diagnostic techniques (general aspects)
Kidney - analysis
Kidney Neoplasms - analysis
Kinetics
Lasers
Luminescence
Medical sciences
Miscellaneous. Technology
Other techniques and industries
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
Porphyrins - analysis
Rats
Rats, Inbred Lew
Spectrometry, Fluorescence - instrumentation
Spectrometry, Fluorescence - methods
Time Factors
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Summary:Steady state fluorescence polarization spectra and time-resolved emission decay kinetics have been measured in vitro from malignant and normal rat kidney tissue. The degrees of polarization and emission lifetimes from the cancerous and normal systems are different. The spectroscopic differences are attributed to environmental transformations local to the native flavin and porphyrin fluorophors' binding sites.
ISSN:0006-3495
1542-0086
DOI:10.1016/S0006-3495(86)83483-X