Molecular aggregation characterized by high order autocorrelation in fluorescence correlation spectroscopy

The use of high order autocorrelation in fluorescence correlation spectroscopy for investigating aggregation in a sample that contains fluorescent molecules is described. Theoretical expressions for the fluorescence fluctuation autocorrelation functions defined by gm,n(tau) = [(delta fm(t + tau)delt...

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Bibliographic Details
Published in:Biophysical journal 1987-08, Vol.52 (2), p.257-270
Main Authors: Palmer, A.G., Thompson, N.L.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Carbocyanines
Fluorescent Dyes
Fundamental and applied biological sciences. Psychology
Intermolecular phenomena
Macromolecular Substances
Mathematics
Miscellaneous
Models, Theoretical
Molecular biophysics
Molecular Conformation
Quinolines
Spectrometry, Fluorescence - methods
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Summary:The use of high order autocorrelation in fluorescence correlation spectroscopy for investigating aggregation in a sample that contains fluorescent molecules is described. Theoretical expressions for the fluorescence fluctuation autocorrelation functions defined by gm,n(tau) = [(delta fm(t + tau)delta fm(t] - (delta Fm(t] (delta Fn(t]]/(F)m+n, where delta F(t) is the fluorescence fluctuation at time t, (F) is the average fluorescence, and m and n are integers less than or equal to 3, are derived. Methods for determining the number densities and relative fluorescence yields of aggregates of different sizes from a series of Gm,n(0) values are outlined. The method is applied to 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate suspended in solutions of water and ethyl alcohol. The technique presented may prove useful in detecting and characterizing aggregates of fluorescent-labeled biological molecules such as cell surface receptors.
ISSN:0006-3495
1542-0086
DOI:10.1016/S0006-3495(87)83213-7