DNA Microarray Analysis of Cyanobacterial Gene Expression during Acclimation to High Light

DNA microarrays bearing nearly all of the genes of the unicellular cyanobacterium Synechocystis sp PCC 6803 were used to examine the temporal program of gene expression during acclimation from low to high light intensity. A complete pattern is provided of gene expression during acclimation of a phot...

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Bibliographic Details
Published in:The Plant cell 2001-04, Vol.13 (4), p.793-806
Main Authors: Hihara, Yukako, Kamei, Ayako, Kanehisa, Minoru, Kaplan, Aaron, Ikeuchi, Masahiko
Format: Article
Language:English
Subjects:
Acclimatization
Adaptation, Physiological
Carbon Dioxide - metabolism
Cyanobacteria - genetics
Cyanobacteria - metabolism
Deoxyribonucleic acid
DNA
DNA microarrays
DNA, Bacterial - analysis
Fluorescent Dyes
Gene expression
Gene Expression Profiling
Gene expression regulation
Gene Expression Regulation, Bacterial
Genes
Light
Light intensity
Luminous intensity
NADH Dehydrogenase - metabolism
Oligonucleotide Array Sequence Analysis
Open Reading Frames
Photochemical reactions
Photochemicals
Photosynthesis
Plant cells
Plants
RNA
Synechocystis
Thylakoids - metabolism
Transcription, Genetic
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Summary:DNA microarrays bearing nearly all of the genes of the unicellular cyanobacterium Synechocystis sp PCC 6803 were used to examine the temporal program of gene expression during acclimation from low to high light intensity. A complete pattern is provided of gene expression during acclimation of a photosynthetic organism to changing light intensity. More than 160 responsive genes were identified and classified into distinct sets. Genes involved in light absorption and photochemical reactions were downregulated within 15 min of exposure to high light intensity, whereas those associated with CO2 fixation and protection from photoinhibition were upregulated. Changes in the expression of genes involved in replication, transcription, and translation, which were induced to support cellular proliferation, occurred later. Several unidentified open reading frames were induced or repressed. The possible involvement of these genes in the acclimation to high light conditions is discussed.
ISSN:1040-4651
1532-298X
DOI:10.1105/tpc.13.4.793