The Role of OsBRI1 and Its Homologous Genes, OsBRL1 and OsBRL3, in Rice

• Published in: Plant physiology (Bethesda) 2006-02, Vol.140 (2), p.580-590
• Main Authors: Nakamura, Ayako, Fujioka, Shozo, Hidehiko Sunohara, Noriko Kamiya, Hong, Zhi, Inukai, Yoshiaki, Kotaro Miura, Takatsuto, Suguru, Yoshida, Shigeo, Ueguchi-Tanaka, Miyako, Yasuko Hasegawa, Kitano, Hidemi, Matsuoka, Makoto
• Format: Article
• Language: English
• Subjects: Alleles; Amino Acid Sequence; Arabidopsis thaliana; Biological and medical sciences; Brassinosteroids; Cell Division - genetics; Cell Enlargement; Development and Hormone Action; Embryos; Fundamental and applied biological sciences. Psychology; Genes. Genome; Leaf primordia; Meristem - cytology; Meristem - metabolism; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; Mutation; Oryza - anatomy & histology; Oryza - growth & development; Oryza - metabolism; Oryza sativa; Phenotype; Phenotypes; Phylogeny; Plant cells; Plant Proteins - genetics; Plant Proteins - physiology; Plant roots; Plant Roots - anatomy & histology; Plant Roots - metabolism; Plants; Receptors; Receptors, Cell Surface - genetics; Receptors, Cell Surface - physiology; Rice; Seeds - cytology; Seeds - growth & development; Seeds - metabolism; Sequence Alignment
• ISSN: 0032-0889; 1532-2548
• DOI: 10.1104/pp.105.072330

Since first identifying two alleles of a rice (Oryza sativa) brassinosteroid (BR)-insensitive mutant, d61, that were also defective in an orthologous gene in Arabidopsis (Arabidopsis thaliana) BRASSINOSTEROID INSENSITIVE1 (BRI1), we have isolated eight additional alleles, including null mutations, of the rice BRI1 gene OsBRI1. The most severe mutant, d61-4, exhibited severe dwarfism and twisted leaves, although pattern formation and differentiation were normal. This severe shoot phenotype was caused mainly by a defect in cell elongation and the disturbance of cell division after the determination of cell fate. In contrast to its severe shoot phenotype, the d61-4 mutant had a mild root phenotype. Concomitantly, the accumulation of castasterone, the active BR in rice, was up to 30-fold greater in the shoots, while only 1.5-fold greater in the roots. The homologous genes for OsBRI1, OsBRL1 and OsBRL3, were highly expressed in roots but weakly expressed in shoots, and their expression was higher in d61-4 than in the wild type. Based on these observations, we conclude that OsBRI1 is not essential for pattern formation or organ initiation, but is involved in organ development through controlling cell division and elongation. In addition, OsBRL1 and OsBRL3 are at least partly involved in BR perception in the roots.