Interaction between Lipid Monolayers and Poloxamer 188: An X-Ray Reflectivity and Diffraction Study

The mechanism by which poloxamer 188 (P188) seals a damaged cell membrane is examined using the lipid monolayer as a model system. X-ray reflectivity and grazing-incidence x-ray diffraction results show that at low nominal lipid density, P188, by physically occupying the available area and phase sep...

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Bibliographic Details
Published in:Biophysical journal 2005-11, Vol.89 (5), p.3159-3173
Main Authors: Wu, Guohui, Majewski, Jaroslaw, Ege, Canay, Kjaer, Kristian, Weygand, Markus Jan, Lee, Ka Yee C.
Format: Article
Language:English
Subjects:
1,2-Dipalmitoylphosphatidylcholine - chemistry
Biophysics
Biophysics - methods
Cell Membrane - metabolism
Diffraction
Electrons
Lipid Bilayers - chemistry
Lipid Metabolism
Lipids
Lipids - chemistry
Membrane Lipids - chemistry
Membranes
Microscopy, Fluorescence
Models, Statistical
Phosphatidylglycerols - chemistry
Phospholipids - chemistry
Poloxamer - chemistry
Pressure
Scattering, Radiation
Surface Properties
Surface-Active Agents - chemistry
Temperature
Time Factors
Water - chemistry
X-Ray Diffraction - methods
X-Rays
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Summary:The mechanism by which poloxamer 188 (P188) seals a damaged cell membrane is examined using the lipid monolayer as a model system. X-ray reflectivity and grazing-incidence x-ray diffraction results show that at low nominal lipid density, P188, by physically occupying the available area and phase separating from the lipids, forces the lipid molecules to pack tightly and restore the barrier function of the membrane. Upon compression to bilayer equivalent pressure, P188 is squeezed out from the lipid monolayer, allowing a graceful exit of P188 when the membrane integrity is restored.
ISSN:0006-3495
1542-0086
DOI:10.1529/biophysj.104.052290