The Complexing of Lysozyme with Poly C and Other homopolymers

Lysozyme forms very large complexes with poly C, in acetate buffer solutions (pH 5.4), when the ratio of lysozyme to poly C concentration is 3/2. When this is less than 3/8 there is virtually no complexing, as evidenced by the low light-scattering power of such mixtures. At such relatively high poly...

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Bibliographic Details
Published in:Biophysical journal 1968-11, Vol.8 (11), p.1201-1210
Main Author: Preiss, John W.
Format: Article
Language:English
Subjects:
Adenine Nucleotides
Chemical Phenomena
Chemistry
Cytosine Nucleotides
Guanine Nucleotides
Hydrogen-Ion Concentration
Molecular Weight
Muramidase
Pancreas - enzymology
Polynucleotides
Ribonucleases
Spectrophotometry
Time Factors
Uracil Nucleotides
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Summary:Lysozyme forms very large complexes with poly C, in acetate buffer solutions (pH 5.4), when the ratio of lysozyme to poly C concentration is 3/2. When this is less than 3/8 there is virtually no complexing, as evidenced by the low light-scattering power of such mixtures. At such relatively high poly C concentrations, the addition of pancreatic ribonuclease causes both the intensity and dissymmetry of scattering to rise to very high values after which time the intensity falls exponentially with time and with very little change in dissymmetry. Other homopolymers also form largest complexes with lysozyme at characteristic concentration ratios.
ISSN:0006-3495
1542-0086
DOI:10.1016/S0006-3495(68)86550-6