RNA secondary structures of the bacteriophage phi6 packaging regions

Bacteriophage phi6 genome consists of three segments of double-stranded RNA. During maturation, single-stranded copies of these segments are packaged into preformed polymerase complex particles. Only phi6 RNA is packaged, and each particle contains only one copy of each segment. An in vitro packagin...

Full description

Saved in:
Bibliographic Details
Published in:RNA (Cambridge) 2000-06, Vol.6 (6), p.880-889
Main Authors: Pirttimaa, M J, Bamford, D H
Format: Article
Language:English
Subjects:
Bacteriophage phi 6 - chemistry
Bacteriophage phi 6 - genetics
Base Sequence
Binding Sites - genetics
Molecular Sequence Data
Nucleic Acid Conformation
Reproducibility of Results
RNA, Viral - chemistry
RNA, Viral - genetics
Sequence Deletion
Virus Assembly - genetics
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Bacteriophage phi6 genome consists of three segments of double-stranded RNA. During maturation, single-stranded copies of these segments are packaged into preformed polymerase complex particles. Only phi6 RNA is packaged, and each particle contains only one copy of each segment. An in vitro packaging and replication assay has been developed for phi6, and the packaging signals (pac sites) have been mapped to the 5' ends of the RNA segments. In this study, we propose secondary structure models for the pac sites of phi6 single-stranded RNA segments. Our models accommodate data from structure-specific chemical modifications, free energy minimizations, and phylogenetic comparisons. Previously reported pac site deletion studies are also discussed. Each pac site possesses a unique architecture, that, however, contains common structural elements.
ISSN:1355-8382
1469-9001