Poly(A)-binding proteins regulate both mRNA deadenylation and decapping in yeast cytoplasmic extracts

The pathway of mRNA degradation has been extensively studied in the yeast, Saccharomyces cerevisiae, and it is now clear that many mRNAs decay by a deadenylation-dependent mechanism. Although several of the factors required for mRNA decay have been identified, the regulation and precise roles of man...

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Bibliographic Details
Published in:RNA (Cambridge) 2001-10, Vol.7 (10), p.1416-1424
Main Authors: WILUSZ, CAROL J., GAO, MIN, JONES, CHARLES L., WILUSZ, JEFFREY, PELTZ, STUART W.
Format: Article
Language:English
Subjects:
Adenine - metabolism
Base Sequence
Cytoplasm - metabolism
DNA Primers
Poly(A)-Binding Proteins
RNA Caps
RNA, Messenger - metabolism
RNA-Binding Proteins - physiology
Saccharomyces cerevisiae - genetics
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Summary:The pathway of mRNA degradation has been extensively studied in the yeast, Saccharomyces cerevisiae, and it is now clear that many mRNAs decay by a deadenylation-dependent mechanism. Although several of the factors required for mRNA decay have been identified, the regulation and precise roles of many of the proteins involved remains unclear. We have developed an in vitro system that recapitulates both the deadenylation and the decapping steps of mRNA decay. Furthermore, both deadenylation and decapping are inhibited by poly(A) binding proteins in our assay. Our system has allowed us to separate the decay process from translation and we have shown that the poly(A) tail is capable of inhibiting decapping in an eIF4E-independent manner. Our in vitro system should prove invaluable in dissecting the mechanisms of mRNA turnover.
ISSN:1355-8382
1469-9001