Cytokine gene expression in Helicobacter pylori associated antral gastritis

Infection of the gastric antrum by Helicobacter pylori is characterised by a cellular inflammatory infiltrate. Whether cytokines are involved in the pathogenesis of this gastritis has been investigated by studying the effect of eradicating H pylori on the expression of genes encoding the cytokines i...

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Bibliographic Details
Published in:Gut 1994-11, Vol.35 (11), p.1567-1570
Main Authors: Moss, S F, Legon, S, Davies, J, Calam, J
Format: Article
Language:English
Subjects:
Adult
Aged
Base Sequence
Blotting, Northern
Duodenal Ulcer - metabolism
Female
Follow-Up Studies
Gastritis - microbiology
Gastritis - pathology
Gene Expression
Helicobacter Infections - metabolism
Helicobacter pylori
Humans
Interleukin-8 - biosynthesis
Intestinal Mucosa - metabolism
Male
Middle Aged
Molecular Sequence Data
Pyloric Antrum - pathology
RNA, Messenger - biosynthesis
Tumor Necrosis Factor-alpha - biosynthesis
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Summary:Infection of the gastric antrum by Helicobacter pylori is characterised by a cellular inflammatory infiltrate. Whether cytokines are involved in the pathogenesis of this gastritis has been investigated by studying the effect of eradicating H pylori on the expression of genes encoding the cytokines interleukin 8 (IL-8) and tumour necrosis factor alpha (TNF-alpha) in the antral mucosa. Gastric antral biopsy specimens were taken from nine patients with duodenal ulcers and cytokine transcripts were identified and quantified by northern blotting. After H pylori had been eradicated the chronic inflammatory infiltrate decreased in all the patients and the polymorphonuclear infiltrate virtually disappeared. Expression of genes also decreased. After eradication, the median TNF-alpha mRNA/rRNA fell to 48% (p = 0.02) and the median IL-8 mRNA/rRNA fell to 5% (p = 0.004) of initial values. These results support the role of increased synthesis of these cytokines in the pathogenesis of the gastritis.
ISSN:0017-5749
1468-3288
1458-3288
DOI:10.1136/gut.35.11.1567