In vitro regulation of thyroglobulin (Tg) autoantibody production by Tg-specific T-cell lines and hybridomas

To define the interactions between self thyroglobulin (Tg)-reactive T and B we co-cultured enriched B cells taken from rat or mouse Tg-primed mice with major histocompatibility complex (MHC) class II-restricted T-cell lines specific for iodinated determinants on self-Tg, or hybridomas derived from t...

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Bibliographic Details
Published in:Immunology 1991-08, Vol.73 (4), p.415-420
Main Authors: CHAMPION, B. R, HUTCHINGS, P, RAYNER, D. C, PAGE, K, TITE, J, COOKE, A, ROITT, I. M
Format: Article
Language:English
Subjects:
Animals
Autoantibodies - biosynthesis
Autoimmunity (experimental aspects and models)
B-Lymphocytes - immunology
Biological and medical sciences
Cell Division - immunology
Cell Line
Cytotoxicity, Immunologic - immunology
Epitopes - immunology
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Hybridomas - immunology
Lymphocyte Cooperation - immunology
Mice
Mice, Inbred CBA
T-Lymphocytes - immunology
Thyroglobulin - immunology
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Summary:To define the interactions between self thyroglobulin (Tg)-reactive T and B we co-cultured enriched B cells taken from rat or mouse Tg-primed mice with major histocompatibility complex (MHC) class II-restricted T-cell lines specific for iodinated determinants on self-Tg, or hybridomas derived from those lines. Using two clonally distinct T-cell hybridomas, ADA2 and CH9, in vitro help for Tg autoantibody responses was observed using mouse (M)Tg-primed B cells and a 100 ng/ml MTg challenge. Using rat Tg-primed B cells and the same conditions, only CH9 provided help, indicating that the fine specificity of B cells influences their ability to interact with specific anti-Tg T-cell clones. In contrast to T-cell hybridomas, their parent T-cell lines MTg9B3 and MTg12B suppressed Tg autoantibody responses in vitro, although they augmented bystander proliferation of unprimed B cells. The MTg12B cells also (i) diminished the survival of Tg-primed B cells, and (ii) inhibited the proliferation of an antigen-presenting B-cell hybridoma (LK35.2) in a cytostasis assay. These findings together support the view that their suppressive activity is mediated through cytotoxicity. While the role of class II-restricted cytotoxic cells in thyroid autoimmunity is unknown, the results suggest that such cells may act to suppress autoantibody responses as well as to mediate tissue damage to class II-expressing thyroid cells.
ISSN:0019-2805
1365-2567