Confirmation of destruction of salmonellae within murine peritoneal exudate cells by immunocytochemical technique

A procedure was developed with which peritoneal exudate cell (PEC) preparations were fixed in a glutaraldehyde-picric acid mixture, post-fixed with osmium tetroxide, embedded in LR White resin and then stained with immunogold probe. It provided tissue sections showing both well-defined ultrastructur...

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Bibliographic Details
Published in:Immunology 1989-07, Vol.67 (3), p.394-400
Main Authors: LIN, F. R, HSU, H. S, MUMAW, V. R, MONCURE, C. W
Format: Article
Language:English
Subjects:
Animals
Antigens, Bacterial - analysis
Ascitic Fluid - immunology
Ascitic Fluid - pathology
Bacterial diseases
Biological and medical sciences
Colloids
Experimental bacterial diseases and models
Gold
Infectious diseases
Macrophages - immunology
Male
Medical sciences
Mice
Mice, Inbred C57BL
Microscopy, Electron
Neutrophils - immunology
Salmonella Infections, Animal - immunology
Salmonella typhimurium
Salmonella typhimurium - immunology
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Summary:A procedure was developed with which peritoneal exudate cell (PEC) preparations were fixed in a glutaraldehyde-picric acid mixture, post-fixed with osmium tetroxide, embedded in LR White resin and then stained with immunogold probe. It provided tissue sections showing both well-defined ultrastructures as well as specifically labelled Salmonella O antigens by electron microscopy. Inbred, male C57BL/6 mice were injected intraperitoneally with 2 x 10(7) virulent Salmonella typhimurium. Peritoneal exudate cells were harvested at 16 and 20 hr after infection. Disintegrating intracellular bacteria were identified as salmonellae by the immunogold markers. Deposition of gold particles in the cytoplasm of phagocytes also indicated that intracellular debris contained digested pathogen. This investigation therefore confirms previous findings of the destruction of salmonellae within inflammatory polymorphs and macrophages.
ISSN:0019-2805
1365-2567