Secretion of interleukin-6 (IL-6)by human monocytes stimulated by muramyl dipeptide and tumour necrosis factor alpha

We studied IL-6 gene expression in human monocytes stimulated by muramyl dipeptide (MDP), a synthetic immunomodulator derived from mycobacterial cell walls. In control monocytes, two IL-6 transcripts of 3.4 kb and 1.6 kb were easily detected at 2.5 hr of culture and remained stable until 18 hr. In M...

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Bibliographic Details
Published in:Immunology 1990, Vol.69 (1), p.52-56
Main Authors: SANCEAU, J, FALCOFF, R, BERANGER, F, CARTER, D. B, WIETZERBIN, J
Format: Article
Language:English
Subjects:
Acetylmuramyl-Alanyl-Isoglutamine - pharmacology
Analysis of the immune response. Humoral and cellular immunity
Biological and medical sciences
Blotting, Northern
Cells, Cultured
Drug Synergism
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Gene Expression - immunology
Humans
Immunobiology
Interleukin-6 - analysis
Interleukin-6 - genetics
Lymphokines, interleukins ( function, expression)
Monocytes - drug effects
Monocytes - immunology
Regulatory factors and their cellular receptors
RNA - analysis
Tumor Necrosis Factor-alpha - pharmacology
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Summary:We studied IL-6 gene expression in human monocytes stimulated by muramyl dipeptide (MDP), a synthetic immunomodulator derived from mycobacterial cell walls. In control monocytes, two IL-6 transcripts of 3.4 kb and 1.6 kb were easily detected at 2.5 hr of culture and remained stable until 18 hr. In MDP-treated monocytes, three IL-6 RNA species displayed different kinetics of accumulation: a 3.4 kb RNA whose expression already reached its maximum after 2.5 hr exposure to MDP; a 1.6 kb RNA whose expression peaked at 5 hr; and a new RNA species of 1.4 kb which was transiently induced in early time of cell stimulation. TNF-alpha co-operated with MDP to increase IL-6 gene expression and secretion of biological active protein (measured by the hybridoma plasmacytoma growth factor assay). MDP exhibits a broad spectrum of immunomodulation properties such as adjuvant activity, enhancement of macrophage cytotoxicity against tumour and induction of non-specific resistance to intracellular agents. The results reported here suggest that these properties might be linked to the stimulation by MDP of genes coding for key cytokines such as IL-6, TNF and IL-1.
ISSN:0019-2805
1365-2567